Gene description for MLC1
Gene name megalencephalic leukoencephalopathy with subcortical cysts 1
Gene symbol MLC1
Other names/aliases LVM
MLC
VL
Species Homo sapiens
 Database cross references - MLC1
ExoCarta ExoCarta_23209
Entrez Gene 23209
HGNC 17082
MIM 605908
UniProt Q15049  
 MLC1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MLC1
Molecular Function
    protein binding GO:0005515 IPI
    transporter activity GO:0005215 NAS
    protein transporter activity GO:0008565 ISS
    protein complex binding GO:0032403 IDA
Biological Process
    positive regulation of intracellular transport GO:0032388 IDA
    cellular response to cholesterol GO:0071397 ISS
    protein oligomerization GO:0051259 IDA
    vesicle-mediated transport GO:0016192 ISS
    regulation of response to osmotic stress GO:0047484 IMP
    caveolin-mediated endocytosis GO:0072584 ISS
    transport GO:0006810 NAS
    ion transport GO:0006811 IEA
    protein transport GO:0015031 IEA
Subcellular Localization
    basolateral plasma membrane GO:0016323 IDA
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    plasma membrane GO:0005886 IDA
    early endosome GO:0005769 ISS
    lysosome GO:0005764 ISS
    integral component of membrane GO:0016021 IDA
    apical plasma membrane GO:0016324 IEA
    recycling endosome GO:0055037 ISS
    endosome GO:0005768 IDA
    clathrin-coated vesicle GO:0030136 ISS
    cell-cell junction GO:0005911 IEA
    caveola GO:0005901 ISS
    membrane raft GO:0045121 ISS
    endoplasmic reticulum GO:0005783 IDA
    cytoplasmic vesicle GO:0031410 IDA
 Experiment description of studies that identified MLC1 in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MLC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MLC1 is involved
No pathways found





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