Gene description for FADS3
Gene name fatty acid desaturase 3
Gene symbol FADS3
Other names/aliases CYB5RP
LLCDL3
Species Homo sapiens
 Database cross references - FADS3
ExoCarta ExoCarta_3995
Vesiclepedia VP_3995
Entrez Gene 3995
HGNC 3576
MIM 606150
UniProt Q9Y5Q0  
 FADS3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FADS3
Molecular Function
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water GO:0016717 IBA
Biological Process
    lipid metabolic process GO:0006629 IBA
    unsaturated fatty acid biosynthetic process GO:0006636 IEA
    unsaturated fatty acid biosynthetic process GO:0006636 NAS
    sphingolipid metabolic process GO:0006665 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 NAS
 Experiment description of studies that identified FADS3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FADS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRC8A 56262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ALG13 79868
Affinity Capture-MS Homo sapiens
3 NPLOC4 55666
Affinity Capture-MS Homo sapiens
4 MFAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 OR10H2  
Affinity Capture-MS Homo sapiens
6 PEX7  
Affinity Capture-MS Homo sapiens
7 YTHDF1 54915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MFSD4  
Affinity Capture-MS Homo sapiens
9 DHRSX 207063
Two-hybrid Homo sapiens
10 IKBIP 121457
Affinity Capture-MS Homo sapiens
11 TMEM186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 FFAR1  
Affinity Capture-MS Homo sapiens
13 PDE2A  
Affinity Capture-MS Homo sapiens
14 FADS2 9415
Affinity Capture-MS Homo sapiens
15 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ERICH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 C19orf26 255057
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SAR1B 51128
Affinity Capture-MS Homo sapiens
19 DHX16 8449
Affinity Capture-MS Homo sapiens
20 GPR182  
Affinity Capture-MS Homo sapiens
21 LPAR2 9170
Affinity Capture-MS Homo sapiens
22 HARS2 23438
Affinity Capture-MS Homo sapiens
23 FNDC3A 22862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 UPF3B 65109
Affinity Capture-MS Homo sapiens
25 ZGPAT  
Affinity Capture-MS Homo sapiens
26 C10orf35  
Affinity Capture-MS Homo sapiens
27 NEMF 9147
Affinity Capture-MS Homo sapiens
28 ALG14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 NAGPA 51172
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PGRMC2 10424
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FADS3 is involved
No pathways found





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