Gene description for ALG13
Gene name ALG13, UDP-N-acetylglucosaminyltransferase subunit
Gene symbol ALG13
Other names/aliases CDG1S
CXorf45
GLT28D1
MDS031
TDRD13
YGL047W
Species Homo sapiens
 Database cross references - ALG13
ExoCarta ExoCarta_79868
Vesiclepedia VP_79868
Entrez Gene 79868
HGNC 30881
MIM 300776
UniProt Q9NP73  
 ALG13 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ALG13
Molecular Function
    N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity GO:0004577 IDA
    N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity GO:0004577 IDA
    N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity GO:0004577 IMP
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 IEA
    protein binding GO:0005515 IPI
Biological Process
    protein N-linked glycosylation GO:0006487 IMP
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IMP
    proteolysis GO:0006508 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    UDP-N-acetylglucosamine transferase complex GO:0043541 IDA
    UDP-N-acetylglucosamine transferase complex GO:0043541 IDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 IGI
 Experiment description of studies that identified ALG13 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ALG13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WDFY3 23001
Affinity Capture-MS Homo sapiens
2 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 EIF4ENIF1 56478
Two-hybrid Homo sapiens
5 YTHDF3  
Two-hybrid Homo sapiens
6 MYCBP 26292
Affinity Capture-MS Homo sapiens
7 Rbm14  
Two-hybrid Mus musculus
8 EXOSC7 23016
Two-hybrid Homo sapiens
9 GRN 2896
Two-hybrid Homo sapiens
10 RHOU 58480
Reconstituted Complex Homo sapiens
11 RBPMS 11030
Two-hybrid Homo sapiens
12 UBE2H 7328
Affinity Capture-MS Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 Rbm38  
Two-hybrid Mus musculus
15 FADS3 3995
Affinity Capture-MS Homo sapiens
16 RERE 473
Two-hybrid Homo sapiens
17 SLC2A4  
Affinity Capture-MS Homo sapiens
18 DMRTB1  
Two-hybrid Homo sapiens
19 ATXN1L  
Two-hybrid Homo sapiens
20 KRT8 3856
Proximity Label-MS Homo sapiens
21 USP36  
Affinity Capture-MS Homo sapiens
22 TRIM66  
Affinity Capture-MS Homo sapiens
23 QKI 9444
Two-hybrid Homo sapiens
24 PLSCR4  
Affinity Capture-MS Homo sapiens
25 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SF1 7536
Two-hybrid Homo sapiens
27 PITX1  
Two-hybrid Homo sapiens
28 MAPKBP1  
Two-hybrid Homo sapiens
29 RPL14 9045
Two-hybrid Homo sapiens
30 PRMT1 3276
Affinity Capture-MS Homo sapiens
31 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RBFOX1 54715
Two-hybrid Homo sapiens
33 NXF2  
Two-hybrid Homo sapiens
34 HOXA1 3198
Two-hybrid Homo sapiens
35 ATN1  
Two-hybrid Homo sapiens
36 PRRC2B  
Two-hybrid Homo sapiens
37 ROR2 4920
Two-hybrid Homo sapiens
38 LATS1  
Proximity Label-MS Homo sapiens
39 ZC3H10  
Two-hybrid Homo sapiens
40 PRPS2 5634
Affinity Capture-MS Homo sapiens
41 ANAPC2 29882
Proximity Label-MS Homo sapiens
42 APEX1 328
Affinity Capture-RNA Homo sapiens
43 PFN1 5216
Proximity Label-MS Homo sapiens
44 RBFOX2 23543
Two-hybrid Homo sapiens
45 CUL9  
Affinity Capture-MS Homo sapiens
46 CDC16 8881
Affinity Capture-MS Homo sapiens
47 EGFL6 25975
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 Ran 19384
Two-hybrid Mus musculus
49 SAV1  
Proximity Label-MS Homo sapiens
50 Bbs5  
Two-hybrid Mus musculus
51 GRSF1 2926
Proximity Label-MS Homo sapiens
52 SERBP1 26135
Proximity Label-MS Homo sapiens
53 CPSF7 79869
Two-hybrid Homo sapiens
54 TRIM52  
Affinity Capture-MS Homo sapiens
55 KRAS 3845
Negative Genetic Homo sapiens
56 LOX 4015
Affinity Capture-MS Homo sapiens
57 FBLN5 10516
Affinity Capture-MS Homo sapiens
58 PCM1 5108
Two-hybrid Homo sapiens
59 CGRRF1  
Affinity Capture-MS Homo sapiens
60 CD63 967
Affinity Capture-MS Homo sapiens
61 TXNL4A 10907
Two-hybrid Homo sapiens
62 KRT31 3881
Two-hybrid Homo sapiens
63 Ssbp1  
Two-hybrid Mus musculus
64 AKAP1 8165
Proximity Label-MS Homo sapiens
65 BAG4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 TULP3 7289
Affinity Capture-MS Homo sapiens
67 ESRP1 54845
Two-hybrid Homo sapiens
68 U2AF1 7307
Two-hybrid Homo sapiens
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