Gene description for Ran
Gene name RAN, member RAS oncogene family
Gene symbol Ran
Other names/aliases -
Species Mus musculus
 Database cross references - Ran
ExoCarta ExoCarta_19384
Entrez Gene 19384
UniProt P62827  
 Ran identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Ran
Molecular Function
    importin-alpha family protein binding GO:0061676 ISO
    poly(A) RNA binding GO:0044822 ISO
    pre-miRNA binding GO:0070883 ISO
    GTPase activity GO:0003924 IBA
    GDP binding GO:0019003 ISO
    protein complex binding GO:0032403 ISO
    protein domain specific binding GO:0019904 ISO
    dynein intermediate chain binding GO:0045505 ISO
    GTP binding GO:0005525 ISO
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
Biological Process
    mitotic nuclear division GO:0007067 IEA
    protein export from nucleus GO:0006611 ISO
    actin cytoskeleton organization GO:0030036 IMP
    cell cycle GO:0007049 IEA
    intracellular protein transport GO:0006886 IEA
    positive regulation of protein binding GO:0032092 ISO
    transport GO:0006810 IEA
    regulation of protein binding GO:0043393 ISO
    cellular protein localization GO:0034613 IMP
    nucleocytoplasmic transport GO:0006913 IEA
    cell division GO:0051301 IEA
    small GTPase mediated signal transduction GO:0007264 IEA
    ribosomal large subunit export from nucleus GO:0000055 ISO
    signal transduction GO:0007165 IEA
    ribosomal subunit export from nucleus GO:0000054 IBA
    protein transport GO:0015031 IEA
    protein import into nucleus, translocation GO:0000060 ISO
    ribosomal small subunit export from nucleus GO:0000056 ISO
    protein localization to nucleolus GO:1902570 ISO
    cellular protein complex localization GO:0034629 IMP
    protein import into nucleus GO:0006606 IDA
Subcellular Localization
    protein complex GO:0043234 ISO
    cell GO:0005623 IBA
    intracellular GO:0005622 IBA
    centriole GO:0005814 ISO
    manchette GO:0002177 ISO
    recycling endosome GO:0055037 ISO
    RNA nuclear export complex GO:0042565 ISO
    nucleus GO:0005634 ISO
    nuclear pore GO:0005643 ISO
    nucleoplasm GO:0005654 ISO
    extracellular exosome GO:0070062 ISO
    chromatin GO:0000785 ISO
    male germ cell nucleus GO:0001673 ISO
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISO
    sperm flagellum GO:0036126 ISO
    midbody GO:0030496 ISO
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Ran in exosomes
1
Experiment ID 26
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ran
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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