Gene description for LRRC8A
Gene name leucine rich repeat containing 8 family, member A
Gene symbol LRRC8A
Other names/aliases AGM5
LRRC8
SWELL1
Species Homo sapiens
 Database cross references - LRRC8A
ExoCarta ExoCarta_56262
Vesiclepedia VP_56262
Entrez Gene 56262
HGNC 19027
MIM 608360
UniProt Q8IWT6  
 LRRC8A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for LRRC8A
Molecular Function
    volume-sensitive anion channel activity GO:0005225 IBA
    volume-sensitive anion channel activity GO:0005225 IDA
    volume-sensitive anion channel activity GO:0005225 IMP
    volume-sensitive anion channel activity GO:0005225 ISS
    monoatomic anion channel activity GO:0005253 IMP
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    cyclic-GMP-AMP transmembrane transporter activity GO:0140360 IDA
Biological Process
    intracellular glucose homeostasis GO:0001678 ISS
    pre-B cell differentiation GO:0002329 ISS
    monoatomic anion transport GO:0006820 IMP
    cell volume homeostasis GO:0006884 IDA
    cell volume homeostasis GO:0006884 IMP
    response to osmotic stress GO:0006970 IMP
    spermatogenesis GO:0007283 ISS
    taurine transmembrane transport GO:0015734 IEA
    aspartate transmembrane transport GO:0015810 IBA
    positive regulation of insulin secretion GO:0032024 ISS
    protein hexamerization GO:0034214 IDA
    protein hexamerization GO:0034214 ISS
    intracellular signal transduction GO:0035556 IBA
    positive regulation of myoblast differentiation GO:0045663 ISS
    monoatomic anion transmembrane transport GO:0098656 IBA
    monoatomic anion transmembrane transport GO:0098656 IDA
    monoatomic anion transmembrane transport GO:0098656 IMP
    monoatomic anion transmembrane transport GO:0098656 ISS
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 IBA
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 IDA
    chloride transmembrane transport GO:1902476 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    lysosomal membrane GO:0005765 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    monoatomic ion channel complex GO:0034702 IBA
    monoatomic ion channel complex GO:0034702 IDA
 Experiment description of studies that identified LRRC8A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LRRC8A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NKAIN1  
Affinity Capture-MS Homo sapiens
2 CRELD1 78987
Affinity Capture-MS Homo sapiens
3 HTR3A  
Affinity Capture-MS Homo sapiens
4 NPEPPS 9520
Affinity Capture-MS Homo sapiens
5 C5AR2  
Affinity Capture-MS Homo sapiens
6 CNGA4  
Affinity Capture-MS Homo sapiens
7 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CLGN 1047
Affinity Capture-MS Homo sapiens
9 CHRNA4  
Affinity Capture-MS Homo sapiens
10 TTYH1  
Affinity Capture-MS Homo sapiens
11 CANX 821
Affinity Capture-MS Homo sapiens
12 SIDT2 51092
Affinity Capture-MS Homo sapiens
13 FADS3 3995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GP9 2815
Affinity Capture-MS Homo sapiens
15 SDHA 6389
Co-fractionation Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SMIM5  
Affinity Capture-MS Homo sapiens
18 KCNK18  
Affinity Capture-MS Homo sapiens
19 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LAMP1 3916
Proximity Label-MS Homo sapiens
21 OR10H2  
Affinity Capture-MS Homo sapiens
22 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 C3AR1  
Affinity Capture-MS Homo sapiens
24 TMEM30B 161291
Affinity Capture-MS Homo sapiens
25 BSCL2  
Affinity Capture-MS Homo sapiens
26 TSPAN15 23555
Affinity Capture-MS Homo sapiens
27 TYROBP  
Affinity Capture-MS Homo sapiens
28 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
29 LGALS9B 284194
Affinity Capture-MS Homo sapiens
30 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
31 HCST  
Affinity Capture-MS Homo sapiens
32 GJA8  
Affinity Capture-MS Homo sapiens
33 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 HSP90AA1 3320
Cross-Linking-MS (XL-MS) Homo sapiens
35 DEFB131  
Affinity Capture-MS Homo sapiens
36 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
37 LPAR1 1902
Affinity Capture-MS Homo sapiens
38 ZDHHC12  
Affinity Capture-MS Homo sapiens
39 FAM69A 388650
Affinity Capture-MS Homo sapiens
40 ATP2A1 487
Proximity Label-MS Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 CMTM5  
Affinity Capture-MS Homo sapiens
43 STS 412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
45 HSP90B2P  
Cross-Linking-MS (XL-MS) Homo sapiens
46 IPPK  
Affinity Capture-MS Homo sapiens
47 KRAS 3845
Proximity Label-MS Homo sapiens
48 Rpl35 66489
Affinity Capture-MS Mus musculus
49 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CD3E 916
Affinity Capture-MS Homo sapiens
51 UPK1A 11045
Affinity Capture-MS Homo sapiens
52 CHRNB1  
Affinity Capture-MS Homo sapiens
53 LRRC8B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 GPR183  
Affinity Capture-MS Homo sapiens
55 ZACN  
Affinity Capture-MS Homo sapiens
56 POPDC2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LRRC8A is involved
PathwayEvidenceSource
Miscellaneous transport and binding events TAS Reactome
Transport of small molecules TAS Reactome





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