Gene description for FADS2
Gene name fatty acid desaturase 2
Gene symbol FADS2
Other names/aliases D6D
DES6
FADSD6
LLCDL2
SLL0262
TU13
Species Homo sapiens
 Database cross references - FADS2
ExoCarta ExoCarta_9415
Vesiclepedia VP_9415
Entrez Gene 9415
HGNC 3575
MIM 606149
UniProt O95864  
 FADS2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FADS2
Molecular Function
    stearoyl-CoA 9-desaturase activity GO:0004768 IEA
    protein binding GO:0005515 IPI
    linoleoyl-CoA desaturase activity GO:0016213 TAS
    oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water GO:0016717 IBA
Biological Process
    arachidonate metabolite production involved in inflammatory response GO:0002538 IEA
    lipid metabolic process GO:0006629 IBA
    unsaturated fatty acid biosynthetic process GO:0006636 IEA
    alpha-linolenic acid metabolic process GO:0036109 TAS
    linoleic acid metabolic process GO:0043651 TAS
    positive regulation of cellular response to oxidative stress GO:1900409 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified FADS2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FADS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOTCH2NL 388677
Two-hybrid Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 PDE2A  
Affinity Capture-MS Homo sapiens
5 RPN2 6185
Proximity Label-MS Homo sapiens
6 FADS3 3995
Affinity Capture-MS Homo sapiens
7 ATXN1L  
Two-hybrid Homo sapiens
8 NBPF19  
Two-hybrid Homo sapiens
9 CUL3 8452
Affinity Capture-MS Homo sapiens
10 EGFR 1956
Affinity Capture-MS Homo sapiens
11 DNAJC25 548645
Proximity Label-MS Homo sapiens
12 ATP1B3 483
Affinity Capture-MS Homo sapiens
13 AMFR 267
Affinity Capture-MS Homo sapiens
14 ERGIC1 57222
Proximity Label-MS Homo sapiens
15 SOX2  
Affinity Capture-MS Homo sapiens
16 TMPO 7112
Affinity Capture-MS Homo sapiens
17 VSIG4  
Affinity Capture-MS Homo sapiens
18 ZNF655  
Two-hybrid Homo sapiens
19 SEC62 7095
Proximity Label-MS Homo sapiens
20 ATP2A1 487
Proximity Label-MS Homo sapiens
21 NTRK1 4914
Affinity Capture-MS Homo sapiens
22 RAB7A 7879
Proximity Label-MS Homo sapiens
23 PINK1  
Affinity Capture-MS Homo sapiens
24 GRSF1 2926
Two-hybrid Homo sapiens
25 KRT40  
Two-hybrid Homo sapiens
26 RPN1 6184
Proximity Label-MS Homo sapiens
27 RAB5C 5878
Proximity Label-MS Homo sapiens
28 AR 367
Affinity Capture-MS Homo sapiens
29 PANK3 79646
Affinity Capture-MS Homo sapiens
30 SEC61B 10952
Proximity Label-MS Homo sapiens
31 DERL1 79139
Proximity Label-MS Homo sapiens
32 RNF139  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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