Gene description for FHIT
Gene name fragile histidine triad
Gene symbol FHIT
Other names/aliases AP3Aase
FRA3B
Species Homo sapiens
 Database cross references - FHIT
ExoCarta ExoCarta_2272
Vesiclepedia VP_2272
Entrez Gene 2272
HGNC 3701
MIM 601153
UniProt P49789  
 FHIT identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for FHIT
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IPI
    adenosine 5'-monophosphoramidase activity GO:0043530 IDA
    adenylylsulfate-ammonia adenylyltransferase activity GO:0047352 IDA
    adenylylsulfatase activity GO:0047627 IDA
    bis(5'-adenosyl)-triphosphatase activity GO:0047710 IBA
    bis(5'-adenosyl)-triphosphatase activity GO:0047710 IDA
Biological Process
    purine nucleotide metabolic process GO:0006163 IBA
    purine nucleotide metabolic process GO:0006163 IDA
    diadenosine triphosphate catabolic process GO:0015964 IBA
    diadenosine triphosphate catabolic process GO:0015964 IDA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IBA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IMP
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IBA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified FHIT in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FHIT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FDCSP  
Affinity Capture-MS Homo sapiens
2 GGH 8836
Affinity Capture-MS Homo sapiens
3 BCAS2 10286
Two-hybrid Homo sapiens
4 RAB40B  
Two-hybrid Homo sapiens
5 TP53 7157
Synthetic Growth Defect Homo sapiens
6 YTHDF1 54915
Two-hybrid Homo sapiens
7 SPERT  
Affinity Capture-MS Homo sapiens
8 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
9 GAST  
Affinity Capture-MS Homo sapiens
10 Rbm14  
Two-hybrid Mus musculus
11 DDIT4L  
Two-hybrid Homo sapiens
12 UBE2I 7329
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
13 TRIM23 373
Two-hybrid Homo sapiens
14 REL 5966
Two-hybrid Homo sapiens
15 CHEK1  
Synthetic Lethality Homo sapiens
16 PRPF6 24148
Two-hybrid Homo sapiens
17 FHIT 2272
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 LEF1  
Affinity Capture-Western Homo sapiens
19 RABL2A 11159
Affinity Capture-MS Homo sapiens
20 MDM2  
Affinity Capture-Western Homo sapiens
21 ARHGAP19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MTMR6 9107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FHIT is involved
No pathways found





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