Gene description for Eif4a1
Gene name eukaryotic translation initiation factor 4A1
Gene symbol Eif4a1
Other names/aliases BM-010
Ddx2a
Eif4
Species Mus musculus
 Database cross references - Eif4a1
ExoCarta ExoCarta_13681
Entrez Gene 13681
UniProt P60843  
 Eif4a1 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Eif4a1
Molecular Function
    helicase activity GO:0004386 IEA
    RNA binding GO:0003723 IEA
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
    hydrolase activity GO:0016787 IEA
    poly(A) RNA binding GO:0044822 ISO
    ATP-dependent RNA helicase activity GO:0004004 IBA
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    translation initiation factor activity GO:0003743 IEA
    double-stranded RNA binding GO:0003725 ISO
Biological Process
    regulation of gene expression GO:0010468 IBA
    translation GO:0006412 IEA
    RNA secondary structure unwinding GO:0010501 IBA
    translational initiation GO:0006413 IBA
Subcellular Localization
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Eif4a1 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Eif4a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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