Gene description for Eif4h
Gene name eukaryotic translation initiation factor 4H
Gene symbol Eif4h
Other names/aliases AU018978
D5Ertd355e
E430026L18Rik
Ef4h
Wbscr1
Wscr1
mKIAA0038
Species Mus musculus
 Database cross references - Eif4h
ExoCarta ExoCarta_22384
Vesiclepedia VP_22384
Entrez Gene 22384
UniProt Q9WUK2  
 Eif4h identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif4h
Molecular Function
    RNA binding GO:0003723 IEA
    translation initiation factor activity GO:0003743 IEA
    RNA strand annealing activity GO:0033592 IBA
    RNA strand-exchange activity GO:0034057 IBA
    ribosomal small subunit binding GO:0043024 IBA
Biological Process
    formation of translation preinitiation complex GO:0001731 IBA
    translational initiation GO:0006413 IEA
    sexual reproduction GO:0019953 IMP
    developmental growth GO:0048589 IMP
    eukaryotic translation initiation factor 4F complex assembly GO:0097010 IBA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Eif4h in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif4h
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Eif4a2 13682
Two-hybrid Mus musculus
3 Fitm2  
Negative Genetic Mus musculus
4 G6pdx 14381
Co-fractionation Mus musculus
5 Unk  
Affinity Capture-RNA Mus musculus
6 Eif4a1 13681
Two-hybrid Mus musculus
View the network image/svg+xml



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