Gene description for Eif4a2
Gene name eukaryotic translation initiation factor 4A2
Gene symbol Eif4a2
Other names/aliases 4833432N07Rik
BM-010
Ddx2b
Eif4
eIF-4A-II
eIF4A-II
Species Mus musculus
 Database cross references - Eif4a2
ExoCarta ExoCarta_13682
Vesiclepedia VP_13682
Entrez Gene 13682
UniProt P10630  
 Eif4a2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Dendritic cells 11390481    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif4a2
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA helicase activity GO:0003724 IEA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IEA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    cytoplasmic translational initiation GO:0002183 IBA
    chromatin remodeling GO:0006338 IEA
    translational initiation GO:0006413 NAS
    chromatin looping GO:0140588 IEA
    negative regulation of RNA-dependent RNA polymerase activity GO:1900260 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    eukaryotic translation initiation factor 4F complex GO:0016281 NAS
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Eif4a2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif4a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 RPL26L1 51121
Two-hybrid Homo sapiens
3 Tsc1  
Affinity Capture-MS Mus musculus
4 NKRF 55922
Two-hybrid Homo sapiens
5 Mapt  
Affinity Capture-MS Mus musculus
6 Atg5  
Affinity Capture-RNA Mus musculus
7 Eif4h 22384
Two-hybrid Mus musculus
8 Unk  
Affinity Capture-RNA Mus musculus
9 DDX24 57062
Two-hybrid Homo sapiens
10 Tcf3  
Affinity Capture-MS Mus musculus
11 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here