Gene description for DUSP4
Gene name dual specificity phosphatase 4
Gene symbol DUSP4
Other names/aliases HVH2
MKP-2
MKP2
TYP
Species Homo sapiens
 Database cross references - DUSP4
ExoCarta ExoCarta_1846
Vesiclepedia VP_1846
Entrez Gene 1846
HGNC 3070
MIM 602747
UniProt Q13115  
 DUSP4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DUSP4
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IBA
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein tyrosine phosphatase activity GO:0004725 TAS
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine/threonine phosphatase activity GO:0008330 IDA
    phosphatase activity GO:0016791 IDA
    myosin phosphatase activity GO:0017018 IEA
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IDA
Biological Process
    endoderm formation GO:0001706 IBA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    signal transduction GO:0007165 IBA
    dephosphorylation GO:0016311 IDA
    negative regulation of MAPK cascade GO:0043409 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified DUSP4 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DUSP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP3S1 1176
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 ACOT7 11332
Affinity Capture-MS Homo sapiens
4 HSD17B4 3295
Affinity Capture-MS Homo sapiens
5 MAP2K6 5608
Affinity Capture-MS Homo sapiens
6 WDR34 89891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ZNF426  
Two-hybrid Homo sapiens
8 MAPK3 5595
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 NUDT10  
Affinity Capture-MS Homo sapiens
10 EIF3C 8663
Affinity Capture-MS Homo sapiens
11 SDHA 6389
Affinity Capture-MS Homo sapiens
12 AMBRA1  
Affinity Capture-MS Homo sapiens
13 MAPK1 5594
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ABHD14B 84836
Affinity Capture-MS Homo sapiens
15 UBE2L3 7332
Affinity Capture-MS Homo sapiens
16 QKI 9444
Affinity Capture-MS Homo sapiens
17 MTCH2 23788
Affinity Capture-MS Homo sapiens
18 NME2P1 283458
Affinity Capture-MS Homo sapiens
19 PRDX5 25824
Affinity Capture-MS Homo sapiens
20 Mapk14  
Affinity Capture-Western Mus musculus
21 PLEKHF2 79666
Two-hybrid Homo sapiens
22 NUP50 10762
Affinity Capture-MS Homo sapiens
23 ACTR1B 10120
Affinity Capture-MS Homo sapiens
24 TNPO3 23534
Affinity Capture-MS Homo sapiens
25 PPIA 5478
Affinity Capture-MS Homo sapiens
26 METTL21B  
Affinity Capture-MS Homo sapiens
27 PARK7 11315
Affinity Capture-MS Homo sapiens
28 C21orf33  
Affinity Capture-MS Homo sapiens
29 APP 351
Reconstituted Complex Homo sapiens
30 VPS29 51699
Affinity Capture-MS Homo sapiens
31 DUSP13  
Affinity Capture-MS Homo sapiens
32 LZTS2 84445
Two-hybrid Homo sapiens
33 DUSP12 11266
Affinity Capture-MS Homo sapiens
34 RNF187  
Affinity Capture-MS Homo sapiens
35 NUDT1  
Affinity Capture-MS Homo sapiens
36 LYSMD1  
Two-hybrid Homo sapiens
37 MAPK9 5601
Co-localization Homo sapiens
38 GORASP2 26003
Two-hybrid Homo sapiens
39 LYSMD2  
Two-hybrid Homo sapiens
40 ZNF655  
Two-hybrid Homo sapiens
41 DUSP4 1846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NOXA1  
Two-hybrid Homo sapiens
43 PRDX6 9588
Affinity Capture-MS Homo sapiens
44 COA7  
Affinity Capture-MS Homo sapiens
45 SRM 6723
Affinity Capture-MS Homo sapiens
46 MAPK14 1432
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
47 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
48 MAPK8 5599
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
49 ENTHD2  
Two-hybrid Homo sapiens
50 HSPB8 26353
Affinity Capture-MS Homo sapiens
51 NXN 64359
Affinity Capture-MS Homo sapiens
52 CRMP1 1400
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DUSP4 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
ERK/MAPK targets TAS Reactome
ERKs are inactivated TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interleukin-17 signaling TAS Reactome
MAP kinase activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK targets/ Nuclear events mediated by MAP kinases TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Nuclear Events (kinase and transcription factor activation) TAS Reactome
RAF-independent MAPK1/3 activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Signal Transduction TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRKs TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome





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