Gene description for WEE1
Gene name WEE1 G2 checkpoint kinase
Gene symbol WEE1
Other names/aliases WEE1A
WEE1hu
Species Homo sapiens
 Database cross references - WEE1
ExoCarta ExoCarta_7465
Entrez Gene 7465
HGNC 12761
MIM 193525
UniProt P30291  
 WEE1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for WEE1
Molecular Function
    protein tyrosine kinase activity GO:0004713 TAS
    magnesium ion binding GO:0000287 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
Biological Process
    cell division GO:0051301 IEA
    blood coagulation GO:0007596 TAS
    establishment of cell polarity GO:0030010 IEA
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    mitotic cell cycle GO:0000278 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    microtubule cytoskeleton organization GO:0000226 IEA
    neuron projection morphogenesis GO:0048812 IEA
    mitotic nuclear division GO:0007067 IEA
    regulation of cell cycle GO:0051726 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
    nucleus GO:0005634 TAS
 Experiment description of studies that identified WEE1 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for WEE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRK 1398
Invivo Homo sapiens
2 PIN1 5300
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 CDCA3 83461
Reconstituted Complex Homo sapiens
5 YWHAB 7529
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here