Gene description for ADA
Gene name adenosine deaminase
Gene symbol ADA
Other names/aliases -
Species Homo sapiens
 Database cross references - ADA
ExoCarta ExoCarta_100
Entrez Gene 100
HGNC 186
MIM 608958
UniProt P00813  
 ADA identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for ADA
Molecular Function
    zinc ion binding GO:0008270 ISS
    adenosine deaminase activity GO:0004000 ISS
    purine nucleoside binding GO:0001883 IEA
    protein binding GO:0005515 IPI
Biological Process
    xanthine biosynthetic process GO:0046111 IEA
    positive regulation of alpha-beta T cell differentiation GO:0046638 IEA
    negative regulation of mature B cell apoptotic process GO:0002906 IEA
    regulation of cell-cell adhesion mediated by integrin GO:0033632 IDA
    positive regulation of B cell proliferation GO:0030890 IEA
    purine ribonucleoside monophosphate biosynthetic process GO:0009168 IEA
    negative regulation of thymocyte apoptotic process GO:0070244 IEA
    lung alveolus development GO:0048286 IEA
    small molecule metabolic process GO:0044281 TAS
    response to vitamin E GO:0033197 IEA
    response to drug GO:0042493 IEA
    negative regulation of inflammatory response GO:0050728 IEA
    response to morphine GO:0043278 IEA
    positive regulation of smooth muscle contraction GO:0045987 IEA
    positive regulation of heart rate GO:0010460 IEA
    germinal center B cell differentiation GO:0002314 IEA
    negative regulation of leukocyte migration GO:0002686 IEA
    response to hydrogen peroxide GO:0042542 IEA
    histamine secretion GO:0001821 IEA
    purine nucleotide salvage GO:0032261 IMP
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    dATP catabolic process GO:0046061 IEA
    positive regulation of T cell differentiation in thymus GO:0033089 IEA
    adenosine catabolic process GO:0006154 ISS
    liver development GO:0001889 IEA
    negative regulation of circadian sleep/wake cycle, non-REM sleep GO:0042323 IEA
    purine nucleobase metabolic process GO:0006144 TAS
    purine-containing compound salvage GO:0043101 TAS
    inosine biosynthetic process GO:0046103 ISS
    positive regulation of germinal center formation GO:0002636 IEA
    T cell activation GO:0042110 IDA
    negative regulation of penile erection GO:0060407 IEA
    embryonic digestive tract development GO:0048566 IEA
    positive regulation of calcium-mediated signaling GO:0050850 IEA
    positive regulation of T cell receptor signaling pathway GO:0050862 IEA
    Peyer's patch development GO:0048541 IEA
    aging GO:0007568 IEA
    placenta development GO:0001890 IEA
    negative regulation of mucus secretion GO:0070256 IEA
    response to hypoxia GO:0001666 IDA
    trophectodermal cell differentiation GO:0001829 IEA
    hypoxanthine salvage GO:0043103 IBA
    negative regulation of adenosine receptor signaling pathway GO:0060169 IDA
    deoxyadenosine catabolic process GO:0006157 IEA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    neuronal cell body GO:0043025 IEA
    dendrite cytoplasm GO:0032839 IEA
    lysosome GO:0005764 IDA
    external side of plasma membrane GO:0009897 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    cell junction GO:0030054 IEA
    extracellular space GO:0005615 IEA
    cytoplasm GO:0005737 IDA
    cell surface GO:0009986 IDA
    cytoplasmic membrane-bounded vesicle lumen GO:0060205 IEA
 Experiment description of studies that identified ADA in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADORA2A  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 ADORA1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
3 NR3C1 2908
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 GRB2 2885
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 DRD1  
Co-localization Homo sapiens
6 DPP4 1803
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which ADA is involved
PathwayEvidenceSource
Purine salvage TAS Reactome





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