Gene description for MPI
Gene name mannose phosphate isomerase
Gene symbol MPI
Other names/aliases CDG1B
PMI
PMI1
Species Homo sapiens
 Database cross references - MPI
ExoCarta ExoCarta_4351
Entrez Gene 4351
HGNC 7216
MIM 154550
UniProt P34949  
 MPI identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MPI
Molecular Function
    mannose-6-phosphate isomerase activity GO:0004476 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    GDP-mannose biosynthetic process GO:0009298 TAS
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified MPI in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MPI
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MPI is involved
PathwayEvidenceSource
Synthesis of GDP-mannose TAS Reactome





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