Gene description for KLHL9
Gene name kelch-like family member 9
Gene symbol KLHL9
Other names/aliases -
Species Homo sapiens
 Database cross references - KLHL9
ExoCarta ExoCarta_55958
Vesiclepedia VP_55958
Entrez Gene 55958
HGNC 18732
MIM 611201
UniProt Q9P2J3  
 KLHL9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for KLHL9
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    cullin family protein binding GO:0097602 IBA
    cullin family protein binding GO:0097602 IPI
Biological Process
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    regulation of cytokinesis GO:0032465 IBA
    regulation of cytokinesis GO:0032465 IMP
    cell division GO:0051301 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    midbody GO:0030496 IDA
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
 Experiment description of studies that identified KLHL9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KLHL9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRN 286262
Affinity Capture-MS Homo sapiens
2 LY86  
Affinity Capture-MS Homo sapiens
3 C1QTNF3 114899
Affinity Capture-MS Homo sapiens
4 ZNF414  
Affinity Capture-MS Homo sapiens
5 MZB1  
Affinity Capture-MS Homo sapiens
6 TMEM44  
Affinity Capture-MS Homo sapiens
7 NXT2  
Affinity Capture-MS Homo sapiens
8 GPS1 2873
Affinity Capture-MS Homo sapiens
9 GIPC1 10755
Affinity Capture-MS Homo sapiens
10 BACH2 60468
Affinity Capture-MS Homo sapiens
11 SOX5 6660
Affinity Capture-MS Homo sapiens
12 USP25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 VIP  
Affinity Capture-MS Homo sapiens
15 TUBA3C 7278
Affinity Capture-MS Homo sapiens
16 WNT3A 89780
Affinity Capture-MS Homo sapiens
17 KIRREL2  
Affinity Capture-MS Homo sapiens
18 IPP  
Affinity Capture-MS Homo sapiens
19 HEXIM1 10614
Affinity Capture-MS Homo sapiens
20 INO80E  
Affinity Capture-MS Homo sapiens
21 TRIM41 90933
Affinity Capture-MS Homo sapiens
22 KLHL26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NUDCD3 23386
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MCFD2 90411
Affinity Capture-MS Homo sapiens
25 CUL3 8452
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
26 ZNF444  
Affinity Capture-MS Homo sapiens
27 KLHL29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 KLHL28  
Affinity Capture-MS Homo sapiens
29 MYH8 4626
Affinity Capture-MS Homo sapiens
30 HEXIM2  
Affinity Capture-MS Homo sapiens
31 KISS1  
Affinity Capture-MS Homo sapiens
32 IGFBP4 3487
Affinity Capture-MS Homo sapiens
33 GJA3  
Affinity Capture-MS Homo sapiens
34 GNLY  
Affinity Capture-MS Homo sapiens
35 FAM219B 57184
Affinity Capture-MS Homo sapiens
36 TMEM171  
Affinity Capture-MS Homo sapiens
37 KLHL13 90293
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SQSTM1 8878
Proximity Label-MS Homo sapiens
39 ATP1A4 480
Affinity Capture-MS Homo sapiens
40 AURKB 9212
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 HOXB1  
Affinity Capture-MS Homo sapiens
43 SYT3  
Affinity Capture-MS Homo sapiens
44 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
45 IGLV6-57  
Affinity Capture-MS Homo sapiens
46 FIBIN  
Affinity Capture-MS Homo sapiens
47 KLHL24  
Affinity Capture-MS Homo sapiens
48 PINK1  
Affinity Capture-MS Homo sapiens
49 COPS6 10980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 NEDD8 4738
Affinity Capture-MS Homo sapiens
51 PLK1 5347
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 COPS5 10987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TPST2 8459
Affinity Capture-MS Homo sapiens
54 BICD2 23299
Proximity Label-MS Homo sapiens
55 SCRIB 23513
Affinity Capture-MS Homo sapiens
56 ETV6  
Affinity Capture-MS Homo sapiens
57 MYH7 4625
Affinity Capture-MS Homo sapiens
58 BHLHE23  
Affinity Capture-MS Homo sapiens
59 NOG 9241
Affinity Capture-MS Homo sapiens
60 MPI 4351
Affinity Capture-MS Homo sapiens
61 FBXL17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 KLHL21 9903
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PCDHGA5  
Affinity Capture-MS Homo sapiens
64 C9orf72  
Affinity Capture-MS Homo sapiens
65 ENC1  
Affinity Capture-MS Homo sapiens
66 SPDEF  
Affinity Capture-MS Homo sapiens
67 CUL4A 8451
Affinity Capture-MS Homo sapiens
68 RBM11  
Affinity Capture-MS Homo sapiens
69 UBE2D1 7321
Reconstituted Complex Homo sapiens
70 KLHL14 57565
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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