Gene description for IGFBP4
Gene name insulin-like growth factor binding protein 4
Gene symbol IGFBP4
Other names/aliases BP-4
HT29-IGFBP
IBP4
IGFBP-4
Species Homo sapiens
 Database cross references - IGFBP4
ExoCarta ExoCarta_3487
Vesiclepedia VP_3487
Entrez Gene 3487
HGNC 5473
MIM 146733
UniProt P22692  
 IGFBP4 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for IGFBP4
Molecular Function
    insulin-like growth factor II binding GO:0031995 IBA
    insulin-like growth factor I binding GO:0031994 IBA
Biological Process
    type B pancreatic cell proliferation GO:0044342 IEA
    cellular protein metabolic process GO:0044267 TAS
    cell proliferation GO:0008283 TAS
    regulation of insulin-like growth factor receptor signaling pathway GO:0043567 IBA
    skeletal system development GO:0001501 TAS
    signal transduction GO:0007165 TAS
    regulation of glucose metabolic process GO:0010906 IEA
    positive regulation of insulin-like growth factor receptor signaling pathway GO:0043568 IEA
    regulation of cell growth GO:0001558 IEA
    DNA metabolic process GO:0006259 TAS
    positive regulation of MAPK cascade GO:0043410 IEA
    inflammatory response GO:0006954 IEA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified IGFBP4 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for IGFBP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGF2 3481
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
2 IGF1  
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
3 TF 7018
Invitro Homo sapiens
4 HK3  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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