Gene description for IGFBP4
Gene name insulin-like growth factor binding protein 4
Gene symbol IGFBP4
Other names/aliases BP-4
HT29-IGFBP
IBP4
IGFBP-4
Species Homo sapiens
 Database cross references - IGFBP4
ExoCarta ExoCarta_3487
Vesiclepedia VP_3487
Entrez Gene 3487
HGNC 5473
MIM 146733
UniProt P22692  
 IGFBP4 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for IGFBP4
Molecular Function
    signaling receptor binding GO:0005102 TAS
    protein binding GO:0005515 IPI
    insulin-like growth factor I binding GO:0031994 IBA
    insulin-like growth factor II binding GO:0031995 IBA
Biological Process
    MAPK cascade GO:0000165 IEA
    regulation of cell growth GO:0001558 IEA
    signal transduction GO:0007165 TAS
    regulation of glucose metabolic process GO:0010906 IEA
    response to organic cyclic compound GO:0014070 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    regulation of insulin-like growth factor receptor signaling pathway GO:0043567 IBA
    positive regulation of insulin-like growth factor receptor signaling pathway GO:0043568 IEA
    type B pancreatic cell proliferation GO:0044342 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    endoplasmic reticulum lumen GO:0005788 TAS
 Experiment description of studies that identified IGFBP4 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for IGFBP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBR2 23304
Affinity Capture-MS Homo sapiens
2 Hk2  
Two-hybrid Rattus norvegicus
Reconstituted Complex Rattus norvegicus
3 RPL13 6137
Affinity Capture-MS Homo sapiens
4 MEGF8 1954
Affinity Capture-MS Homo sapiens
5 FBXO45 200933
Affinity Capture-MS Homo sapiens
6 IL17RA 23765
Affinity Capture-MS Homo sapiens
7 MYCBP2 23077
Affinity Capture-MS Homo sapiens
8 KLHL9 55958
Affinity Capture-MS Homo sapiens
9 NUDT12  
Affinity Capture-MS Homo sapiens
10 NOTCH2 4853
Affinity Capture-MS Homo sapiens
11 KLHL26  
Affinity Capture-MS Homo sapiens
12 MEX3A  
Affinity Capture-RNA Homo sapiens
Protein-RNA Homo sapiens
13 RPL3 6122
Affinity Capture-MS Homo sapiens
14 CETN3  
Affinity Capture-MS Homo sapiens
15 IGF1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
17 NISCH 11188
Affinity Capture-MS Homo sapiens
18 SBDS 51119
Affinity Capture-MS Homo sapiens
19 KLHL13 90293
Affinity Capture-MS Homo sapiens
20 LONP2 83752
Affinity Capture-MS Homo sapiens
21 VWDE  
Affinity Capture-MS Homo sapiens
22 KDM1A 23028
Two-hybrid Homo sapiens
23 SPRYD3 84926
Affinity Capture-MS Homo sapiens
24 KLHL22 84861
Affinity Capture-MS Homo sapiens
25 IMPDH2 3615
Affinity Capture-MS Homo sapiens
26 P3H3 10536
Affinity Capture-MS Homo sapiens
27 SUV39H1  
Two-hybrid Homo sapiens
28 BMP1 649
Affinity Capture-MS Homo sapiens
29 Hk3  
Two-hybrid Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Reconstituted Complex Rattus norvegicus
30 NOTCH1 4851
Affinity Capture-MS Homo sapiens
31 IGF2 3481
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
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