Gene description for P3H3
Gene name prolyl 3-hydroxylase 3
Gene symbol P3H3
Other names/aliases GRCB
HSU47926
LEPREL2
Species Homo sapiens
 Database cross references - P3H3
ExoCarta ExoCarta_10536
Vesiclepedia VP_10536
Entrez Gene 10536
HGNC 19318
MIM 610342
UniProt Q8IVL6  
 P3H3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for P3H3
Molecular Function
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    procollagen-proline 3-dioxygenase activity GO:0019797 IBA
    L-ascorbic acid binding GO:0031418 IEA
Biological Process
    negative regulation of cell population proliferation GO:0008285 IDA
    peptidyl-lysine hydroxylation GO:0017185 ISS
    collagen metabolic process GO:0032963 IBA
    collagen biosynthetic process GO:0032964 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    catalytic complex GO:1902494 ISS
 Experiment description of studies that identified P3H3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for P3H3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 HSPA5 3309
Affinity Capture-MS Homo sapiens
4 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CPSF6 11052
Affinity Capture-MS Homo sapiens
6 CA6 765
Affinity Capture-MS Homo sapiens
7 EPDR1 54749
Affinity Capture-MS Homo sapiens
8 ADAMTS4  
Affinity Capture-MS Homo sapiens
9 CEACAM8 1088
Affinity Capture-MS Homo sapiens
10 LGI1  
Affinity Capture-MS Homo sapiens
11 KRTAP1-3  
Two-hybrid Homo sapiens
12 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 EMILIN3  
Affinity Capture-MS Homo sapiens
14 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LRRC59 55379
Proximity Label-MS Homo sapiens
16 DCAF8L2  
Affinity Capture-MS Homo sapiens
17 PDIA4 9601
Proximity Label-MS Homo sapiens
18 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 C1QTNF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GGH 8836
Affinity Capture-MS Homo sapiens
21 NCR3  
Affinity Capture-MS Homo sapiens
22 PTCH1  
Affinity Capture-MS Homo sapiens
23 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PDGFRA 5156
Affinity Capture-MS Homo sapiens
25 FASLG 356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PRG2  
Affinity Capture-MS Homo sapiens
27 HRG 3273
Affinity Capture-MS Homo sapiens
28 PI15  
Affinity Capture-MS Homo sapiens
29 RLN1  
Affinity Capture-MS Homo sapiens
30 CBLN4  
Affinity Capture-MS Homo sapiens
31 C1QTNF8  
Affinity Capture-MS Homo sapiens
32 TRIM55  
Two-hybrid Homo sapiens
33 IGFBP4 3487
Affinity Capture-MS Homo sapiens
34 COLQ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SOCS7  
Affinity Capture-MS Homo sapiens
37 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
39 KRTAP12-3  
Two-hybrid Homo sapiens
40 SFTPA2  
Affinity Capture-MS Homo sapiens
41 IDE 3416
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C1QTNF9B  
Affinity Capture-MS Homo sapiens
43 C1QB 713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 MRPS18B 28973
Affinity Capture-MS Homo sapiens
45 SIRPD  
Affinity Capture-MS Homo sapiens
46 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 GIP  
Affinity Capture-MS Homo sapiens
48 C1QA 712
Affinity Capture-MS Homo sapiens
49 FIBIN  
Affinity Capture-MS Homo sapiens
50 RTN4R 65078
Affinity Capture-MS Homo sapiens
51 EPHA7 2045
Affinity Capture-MS Homo sapiens
52 ANKDD1A  
Affinity Capture-MS Homo sapiens
53 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 C1orf54  
Affinity Capture-MS Homo sapiens
55 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
56 ALPP 250
Affinity Capture-MS Homo sapiens
57 DNAJB9 4189
Affinity Capture-MS Homo sapiens
58 KNG1 3827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 KIRREL2  
Affinity Capture-MS Homo sapiens
60 BRD1 23774
Affinity Capture-MS Homo sapiens
61 PRR4 11272
Affinity Capture-MS Homo sapiens
62 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 DHH  
Affinity Capture-MS Homo sapiens
64 KRTAP9-2  
Two-hybrid Homo sapiens
65 EDN3  
Affinity Capture-MS Homo sapiens
66 OLFM4 10562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 THEG  
Affinity Capture-MS Homo sapiens
68 MFAP4 4239
Affinity Capture-MS Homo sapiens
69 PRELP 5549
Affinity Capture-MS Homo sapiens
70 CFC1  
Affinity Capture-MS Homo sapiens
71 SDF2L1 23753
Affinity Capture-MS Homo sapiens
72 IL5RA  
Affinity Capture-MS Homo sapiens
73 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
74 C1QTNF7  
Affinity Capture-MS Homo sapiens
75 TRIM63  
Two-hybrid Homo sapiens
76 LIPG 9388
Affinity Capture-MS Homo sapiens
77 KEAP1 9817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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