Gene description for DENND4C
Gene name DENN/MADD domain containing 4C
Gene symbol DENND4C
Other names/aliases C9orf55
C9orf55B
bA513M16.3
Species Homo sapiens
 Database cross references - DENND4C
ExoCarta ExoCarta_55667
Vesiclepedia VP_55667
Entrez Gene 55667
HGNC 26079
UniProt Q5VZ89  
 DENND4C identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DENND4C
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
Biological Process
    protein transport GO:0015031 IEA
    regulation of Rab protein signal transduction GO:0032483 IBA
    cellular response to insulin stimulus GO:0032869 ISS
    protein localization to plasma membrane GO:0072659 ISS
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISS
    cytoplasmic vesicle membrane GO:0030659 IEA
    retromer complex GO:0030904 IEA
    cytoplasmic vesicle GO:0031410 IBA
    insulin-responsive compartment GO:0032593 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified DENND4C in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DENND4C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VPS35 55737
Affinity Capture-MS Homo sapiens
2 HDAC4  
Affinity Capture-MS Homo sapiens
3 RPTOR 57521
Affinity Capture-MS Homo sapiens
4 KSR1  
Affinity Capture-MS Homo sapiens
5 RALGPS2 55103
Affinity Capture-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 GJA1 2697
Proximity Label-MS Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 NAV1 89796
Affinity Capture-MS Homo sapiens
10 CDC25B 994
Affinity Capture-MS Homo sapiens
11 SRGAP2 23380
Affinity Capture-MS Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CAMSAP2  
Affinity Capture-MS Homo sapiens
15 CBY1  
Affinity Capture-MS Homo sapiens
16 EIF4E2  
Affinity Capture-MS Homo sapiens
17 C1orf112  
Affinity Capture-MS Homo sapiens
18 Ecd  
Affinity Capture-MS Mus musculus
19 KIF13B 23303
Affinity Capture-MS Homo sapiens
20 Pqbp1  
Affinity Capture-MS Mus musculus
21 Tgs1  
Affinity Capture-MS Mus musculus
22 HSPA2 3306
Affinity Capture-MS Homo sapiens
23 ZBTB21  
Affinity Capture-MS Homo sapiens
24 RASAL2 9462
Affinity Capture-MS Homo sapiens
25 ZNF638 27332
Affinity Capture-MS Homo sapiens
26 CALM3 808
Affinity Capture-MS Homo sapiens
27 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TIAM1  
Affinity Capture-MS Homo sapiens
29 PHLDB2 90102
Affinity Capture-MS Homo sapiens
30 STX6 10228
Proximity Label-MS Homo sapiens
31 PTPN14 5784
Affinity Capture-MS Homo sapiens
32 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LRFN1  
Affinity Capture-MS Homo sapiens
34 RAB35 11021
Proximity Label-MS Homo sapiens
35 EBAG9 9166
Proximity Label-MS Homo sapiens
36 KIAA1804  
Affinity Capture-MS Homo sapiens
37 LAMTOR1 55004
Proximity Label-MS Homo sapiens
38 MELK  
Affinity Capture-MS Homo sapiens
39 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 AGAP1  
Affinity Capture-MS Homo sapiens
41 PFN1 5216
Proximity Label-MS Homo sapiens
42 CALM2 805
Affinity Capture-MS Homo sapiens
43 RAB5A 5868
Proximity Label-MS Homo sapiens
44 CDK16 5127
Affinity Capture-MS Homo sapiens
45 ANKRD34A  
Affinity Capture-MS Homo sapiens
46 USP21  
Affinity Capture-MS Homo sapiens
47 TGM2 7052
Affinity Capture-MS Homo sapiens
48 FAM110A  
Affinity Capture-MS Homo sapiens
49 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
50 TESK2  
Affinity Capture-MS Homo sapiens
51 LYN 4067
Proximity Label-MS Homo sapiens
52 RAB4A 5867
Proximity Label-MS Homo sapiens
53 CDH1 999
Proximity Label-MS Homo sapiens
54 YWHAE 7531
Affinity Capture-MS Homo sapiens
55 LPIN3 64900
Affinity Capture-MS Homo sapiens
56 YWHAQ 10971
Affinity Capture-MS Homo sapiens
57 FAM110B  
Affinity Capture-MS Homo sapiens
58 TANC2  
Affinity Capture-MS Homo sapiens
59 KCTD3  
Affinity Capture-MS Homo sapiens
60 PIPSL 266971
Affinity Capture-MS Homo sapiens
61 PHLPP1  
Proximity Label-MS Homo sapiens
62 LIMA1 51474
Affinity Capture-MS Homo sapiens
63 DCLK1 9201
Affinity Capture-MS Homo sapiens
64 XPO1 7514
Affinity Capture-MS Homo sapiens
65 LAMP1 3916
Proximity Label-MS Homo sapiens
66 OSBPL6  
Affinity Capture-MS Homo sapiens
67 INPP5E 56623
Affinity Capture-MS Homo sapiens
68 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ARF6 382
Proximity Label-MS Homo sapiens
70 NADK 65220
Affinity Capture-MS Homo sapiens
71 DUSP16  
Affinity Capture-MS Homo sapiens
72 WEE1 7465
Affinity Capture-MS Homo sapiens
73 SYDE1 85360
Affinity Capture-MS Homo sapiens
74 CDC25C  
Affinity Capture-MS Homo sapiens
75 PTPN13 5783
Affinity Capture-MS Homo sapiens
76 GIGYF1  
Affinity Capture-MS Homo sapiens
77 RAB9A 9367
Proximity Label-MS Homo sapiens
78 GOLGA1  
Proximity Label-MS Homo sapiens
79 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
80 CGN  
Affinity Capture-MS Homo sapiens
81 SH3RF3  
Affinity Capture-MS Homo sapiens
82 GIGYF2 26058
Affinity Capture-MS Homo sapiens
83 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
84 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 STARD13 90627
Affinity Capture-MS Homo sapiens
86 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
87 FAM53C 51307
Affinity Capture-MS Homo sapiens
88 CDK8 1024
Affinity Capture-MS Homo sapiens
89 RTKN 6242
Affinity Capture-MS Homo sapiens
90 DENND1A 57706
Affinity Capture-MS Homo sapiens
91 PPM1H  
Affinity Capture-MS Homo sapiens
92 SRSF12  
Affinity Capture-MS Homo sapiens
93 AGPS 8540
Proximity Label-MS Homo sapiens
94 GBA 2629
Affinity Capture-MS Homo sapiens
95 GAB2 9846
Affinity Capture-MS Homo sapiens
96 NF1 4763
Affinity Capture-MS Homo sapiens
97 RAB2A 5862
Proximity Label-MS Homo sapiens
98 DENND4A 10260
Affinity Capture-MS Homo sapiens
99 RAB7A 7879
Proximity Label-MS Homo sapiens
100 RAB11A 8766
Proximity Label-MS Homo sapiens
101 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
102 KIF1C 10749
Affinity Capture-MS Homo sapiens
103 MAST3  
Affinity Capture-MS Homo sapiens
104 MAGI1  
Affinity Capture-MS Homo sapiens
105 CAV1 857
Proximity Label-MS Homo sapiens
106 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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