Gene description for RRM2
Gene name ribonucleotide reductase M2
Gene symbol RRM2
Other names/aliases R2
RR2
RR2M
Species Homo sapiens
 Database cross references - RRM2
ExoCarta ExoCarta_6241
Vesiclepedia VP_6241
Entrez Gene 6241
HGNC 10452
MIM 180390
UniProt P31350  
 RRM2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
 Gene ontology annotations for RRM2
Molecular Function
    protein binding GO:0005515 IPI
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 NAS
    metal ion binding GO:0046872 IEA
Biological Process
    mitotic cell cycle GO:0000278 TAS
    nucleobase-containing small molecule interconversion GO:0015949 TAS
    oxidation-reduction process GO:0055114 IEA
    protein heterotetramerization GO:0051290 IEA
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0000083 TAS
    small molecule metabolic process GO:0044281 TAS
    DNA replication GO:0006260 IEA
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    deoxyribonucleoside diphosphate metabolic process GO:0009186 IEA
    deoxyribonucleotide biosynthetic process GO:0009263 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RRM2 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 236
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 260
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RRM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 RRM1 6240
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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