Gene description for RRM2
Gene name ribonucleotide reductase M2
Gene symbol RRM2
Other names/aliases R2
RR2
RR2M
Species Homo sapiens
 Database cross references - RRM2
ExoCarta ExoCarta_6241
Vesiclepedia VP_6241
Entrez Gene 6241
HGNC 10452
MIM 180390
UniProt P31350  
 RRM2 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
 Gene ontology annotations for RRM2
Molecular Function
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 IBA
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 ISS
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 NAS
    protein binding GO:0005515 IPI
    ferric iron binding GO:0008199 IEA
    protein homodimerization activity GO:0042803 IEA
Biological Process
    blastocyst development GO:0001824 IEA
    ribonucleoside diphosphate metabolic process GO:0009185 IDA
    deoxyribonucleotide biosynthetic process GO:0009263 IBA
    deoxyribonucleotide biosynthetic process GO:0009263 ISS
    2'-deoxyribonucleotide biosynthetic process GO:0009265 IDA
    protein heterotetramerization GO:0051290 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ribonucleoside-diphosphate reductase complex GO:0005971 IPI
 Experiment description of studies that identified RRM2 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RRM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDHAF2 54949
Affinity Capture-MS Homo sapiens
2 DNAAF5 54919
Co-fractionation Homo sapiens
3 Anapc2  
Affinity Capture-MS Mus musculus
4 SPAG9 9043
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 SDCBP 6386
Two-hybrid Homo sapiens
7 NPLOC4 55666
Co-fractionation Homo sapiens
8 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PARP1 142
Negative Genetic Homo sapiens
10 PIK3CA 5290
Negative Genetic Homo sapiens
11 ARID2  
Negative Genetic Homo sapiens
12 Cdc23  
Affinity Capture-MS Mus musculus
13 TP53 7157
Affinity Capture-Western Homo sapiens
14 HSPA8 3312
Affinity Capture-MS Homo sapiens
15 BAG2 9532
Affinity Capture-MS Homo sapiens
16 ASNS 440
Co-fractionation Homo sapiens
17 RBMX 27316
Co-fractionation Homo sapiens
18 MAPK1 5594
Co-fractionation Homo sapiens
19 PITHD1 57095
Affinity Capture-MS Homo sapiens
20 KAT5  
Reconstituted Complex Homo sapiens
21 TMEM248 55069
Affinity Capture-MS Homo sapiens
22 ANAPC15  
Affinity Capture-MS Homo sapiens
23 HRNR 388697
Affinity Capture-MS Homo sapiens
24 PDGFRA 5156
Negative Genetic Homo sapiens
25 CDC23 8697
Affinity Capture-MS Homo sapiens
26 SKP2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
27 SQSTM1 8878
Proximity Label-MS Homo sapiens
28 HSPA1A 3303
Affinity Capture-MS Homo sapiens
29 EGFR 1956
Negative Genetic Homo sapiens
30 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
31 INPPL1 3636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NUP43 348995
Proximity Label-MS Homo sapiens
33 CCNF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 DDX19B 11269
Co-fractionation Homo sapiens
35 CREB1  
Affinity Capture-MS Homo sapiens
36 VHL  
Negative Genetic Homo sapiens
37 C17orf85  
Affinity Capture-MS Homo sapiens
38 PRKDC 5591
Negative Genetic Homo sapiens
39 WEE1 7465
Negative Genetic Homo sapiens
40 NONO 4841
Co-fractionation Homo sapiens
41 MAP7D3 79649
Co-fractionation Homo sapiens
42 Cdc16  
Affinity Capture-MS Mus musculus
43 FZR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 NR1H4  
Affinity Capture-Western Homo sapiens
45 RPS6KB1 6198
Negative Genetic Homo sapiens
46 CDC16 8881
Affinity Capture-MS Homo sapiens
47 RPA3 6119
Proximity Label-MS Homo sapiens
48 SEC31A 22872
Co-fractionation Homo sapiens
49 DYRK1A 1859
Affinity Capture-MS Homo sapiens
50 RRM1 6240
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 PPP2R5D 5528
Co-fractionation Homo sapiens
53 CDK9 1025
Negative Genetic Homo sapiens
54 ANAPC4 29945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PLK1 5347
Co-fractionation Homo sapiens
56 RRM2B 50484
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ERBB2 2064
Negative Genetic Homo sapiens
58 SIRT6  
Affinity Capture-MS Homo sapiens
59 BICD2 23299
Proximity Label-MS Homo sapiens
60 GDI1 2664
Co-fractionation Homo sapiens
61 KRAS 3845
Negative Genetic Homo sapiens
62 C9orf72  
Affinity Capture-MS Homo sapiens
63 AR 367
Affinity Capture-MS Homo sapiens
64 CDC27 996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 LMNA 4000
Proximity Label-MS Homo sapiens
66 CSTF1 1477
Co-fractionation Homo sapiens
67 PCMT1 5110
Co-fractionation Homo sapiens
68 NCCRP1 342897
Affinity Capture-MS Homo sapiens
69 DNM1L 10059
Two-hybrid Homo sapiens
70 CDKN2A 1029
Negative Genetic Homo sapiens
71 Cdc26  
Affinity Capture-MS Mus musculus
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