Gene description for ALG3
Gene name ALG3, alpha-1,3- mannosyltransferase
Gene symbol ALG3
Other names/aliases CDG1D
CDGS4
D16Ertd36e
NOT56L
Not56
Species Homo sapiens
 Database cross references - ALG3
ExoCarta ExoCarta_10195
Vesiclepedia VP_10195
Entrez Gene 10195
HGNC 23056
MIM 608750
UniProt Q92685  
 ALG3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ALG3
Molecular Function
    alpha-1,3-mannosyltransferase activity GO:0000033 TAS
    protein binding GO:0005515 IPI
    dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity GO:0052925 IBA
    dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity GO:0052925 IDA
Biological Process
    protein glycosylation GO:0006486 IBA
    protein N-linked glycosylation GO:0006487 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    lumenal side of endoplasmic reticulum membrane GO:0098553 IC
 Experiment description of studies that identified ALG3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ALG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ERGIC3 51614
Affinity Capture-MS Homo sapiens
2 TMEM52B 120939
Two-hybrid Homo sapiens
3 C5AR1  
Affinity Capture-MS Homo sapiens
4 FASN 2194
Negative Genetic Homo sapiens
5 VIPR2  
Affinity Capture-MS Homo sapiens
6 SSMEM1  
Two-hybrid Homo sapiens
7 TSPAN31 6302
Affinity Capture-MS Homo sapiens
8 CLDND1 56650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SYNGR1 9145
Affinity Capture-MS Homo sapiens
10 TSGA10IP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GFAP 2670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CRELD1 78987
Affinity Capture-MS Homo sapiens
13 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
14 TSPAN15 23555
Affinity Capture-MS Homo sapiens
15 DBNL 28988
Affinity Capture-MS Homo sapiens
16 GCGR  
Affinity Capture-MS Homo sapiens
17 NIPAL1  
Affinity Capture-MS Homo sapiens
18 CD79A  
Two-hybrid Homo sapiens
19 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GPR52  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C12orf49  
Negative Genetic Homo sapiens
22 CREB3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 CMTM5  
Affinity Capture-MS Homo sapiens
24 GPR21  
Affinity Capture-MS Homo sapiens
25 ALDH3B1 221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 LDLR 3949
Negative Genetic Homo sapiens
27 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
28 CCR6  
Affinity Capture-MS Homo sapiens
29 ARL13B 200894
Two-hybrid Homo sapiens
30 SLC22A4 6583
Affinity Capture-MS Homo sapiens
31 SERP1 27230
Two-hybrid Homo sapiens
32 TTYH1  
Affinity Capture-MS Homo sapiens
33 CD93  
Affinity Capture-MS Homo sapiens
34 AVPR2  
Affinity Capture-MS Homo sapiens
35 KLHL14 57565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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