Gene description for PSEN1
Gene name presenilin 1
Gene symbol PSEN1
Other names/aliases AD3
FAD
PS-1
PS1
S182
Species Homo sapiens
 Database cross references - PSEN1
ExoCarta ExoCarta_5663
Vesiclepedia VP_5663
Entrez Gene 5663
HGNC 9508
MIM 104311
UniProt P49768  
 PSEN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PSEN1
Molecular Function
    beta-catenin binding GO:0008013 IPI
    PDZ domain binding GO:0030165 IPI
    cadherin binding GO:0045296 IBA
    calcium channel activity GO:0005262 IMP
    endopeptidase activity GO:0004175 IDA
    protein binding GO:0005515 IPI
    aspartic-type endopeptidase activity GO:0004190 IEA
Biological Process
    regulation of protein binding GO:0043393 IEA
    neuron development GO:0048666 IEA
    positive regulation of MAP kinase activity GO:0043406 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
    Cajal-Retzius cell differentiation GO:0021870 IEA
    neuron migration GO:0001764 IEA
    dorsal/ventral neural tube patterning GO:0021904 IEA
    epithelial cell proliferation GO:0050673 IEA
    negative regulation of epidermal growth factor-activated receptor activity GO:0007175 IEA
    negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000059 IEA
    T cell activation involved in immune response GO:0002286 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    choline transport GO:0015871 IEA
    embryonic limb morphogenesis GO:0030326 IEA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    mitochondrial transport GO:0006839 IEA
    brain morphogenesis GO:0048854 IEA
    T cell receptor signaling pathway GO:0050852 IEA
    positive regulation of receptor recycling GO:0001921 IEA
    positive regulation of coagulation GO:0050820 IEA
    skeletal system morphogenesis GO:0048705 IEA
    post-embryonic development GO:0009791 IEA
    regulation of resting membrane potential GO:0060075 IEA
    positive regulation of dendritic spine development GO:0060999 IMP
    positive regulation of catalytic activity GO:0043085 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    endoplasmic reticulum calcium ion homeostasis GO:0032469 IGI
    response to oxidative stress GO:0006979 IEA
    Notch signaling pathway GO:0007219 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    intracellular signal transduction GO:0035556 IEA
    Notch receptor processing GO:0007220 TAS
    skin morphogenesis GO:0043589 IEA
    autophagosome assembly GO:0000045 IEA
    regulation of synaptic transmission, glutamatergic GO:0051966 IEA
    cellular response to DNA damage stimulus GO:0006974 IEA
    synaptic vesicle targeting GO:0016080 IEA
    extracellular matrix disassembly GO:0022617 TAS
    cerebral cortex cell migration GO:0021795 IEA
    extracellular matrix organization GO:0030198 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    activation of MAPKK activity GO:0000186 IEA
    regulation of synaptic plasticity GO:0048167 IEA
    L-glutamate transport GO:0015813 IEA
    myeloid dendritic cell differentiation GO:0043011 IEA
    beta-amyloid formation GO:0034205 IEA
    protein glycosylation GO:0006486 IEA
    memory GO:0007613 IEA
    regulation of phosphorylation GO:0042325 IDA
    amyloid precursor protein catabolic process GO:0042987 TAS
    calcium ion transmembrane transport GO:0070588 IMP
    negative regulation of ubiquitin-protein transferase activity GO:0051444 IEA
    heart looping GO:0001947 IEA
    canonical Wnt signaling pathway GO:0060070 IBA
    protein transport GO:0015031 IEA
    neuron apoptotic process GO:0051402 IEA
    blood vessel development GO:0001568 IEA
    negative regulation of axonogenesis GO:0050771 IEA
    protein processing GO:0016485 IDA
    positive regulation of apoptotic process GO:0043065 IEA
    smooth endoplasmic reticulum calcium ion homeostasis GO:0051563 IBA
    beta-amyloid metabolic process GO:0050435 IBA
    somitogenesis GO:0001756 IEA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    single organismal cell-cell adhesion GO:0016337 IMP
    thymus development GO:0048538 IEA
    cell fate specification GO:0001708 IEA
Subcellular Localization
    neuronal cell body GO:0043025 IBA
    endoplasmic reticulum GO:0005783 IDA
    dendritic shaft GO:0043198 IBA
    cell cortex GO:0005938 IBA
    Golgi membrane GO:0000139 IEA
    growth cone GO:0030426 IBA
    ciliary rootlet GO:0035253 IBA
    kinetochore GO:0000776 IDA
    perinuclear region of cytoplasm GO:0048471 IBA
    centrosome GO:0005813 IDA
    mitochondrion GO:0005739 IDA
    membrane GO:0016020 IDA
    Golgi apparatus GO:0005794 IDA
    integral component of plasma membrane GO:0005887 IDA
    mitochondrial inner membrane GO:0005743 IBA
    plasma membrane GO:0005886 IDA
    axon GO:0030424 IBA
    membrane raft GO:0045121 IDA
    cell surface GO:0009986 IBA
    nuclear membrane GO:0031965 IDA
    Z disc GO:0030018 IBA
    lysosomal membrane GO:0005765 IBA
    aggresome GO:0016235 IDA
    nuclear outer membrane GO:0005640 IDA
    integral component of membrane GO:0016021 TAS
    smooth endoplasmic reticulum GO:0005790 IDA
    gamma-secretase complex GO:0070765 IDA
    rough endoplasmic reticulum GO:0005791 IDA
    apical plasma membrane GO:0016324 IBA
    neuromuscular junction GO:0031594 IBA
    cytoplasmic vesicle GO:0031410 IEA
    nucleus GO:0005634 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
 Experiment description of studies that identified PSEN1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PSEN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSEN2 5664
Affinity Capture-Western Homo sapiens
2 ERN1  
Invivo Homo sapiens
Invitro Homo sapiens
3 PSEN1 5663
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 NCSTN 23385
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 Ctnnb1 12387
Affinity Capture-Western Mus musculus
6 CASP7  
Affinity Capture-MS Homo sapiens
7 CTNND2 1501
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 PSENEN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 GFAP 2670
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 TCF7L2  
Invitro Homo sapiens
12 CLSTN1 22883
Affinity Capture-Western Homo sapiens
13 METTL2B 55798
Two-hybrid Homo sapiens
14 APP 351
Affinity Capture-MS Homo sapiens
15 FBXW7  
Affinity Capture-Western Homo sapiens
16 MAPT  
Invivo Homo sapiens
17 CDH1 999
Affinity Capture-Western Homo sapiens
18 YME1L1 10730
Two-hybrid Homo sapiens
19 PKP4 8502
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
20 CASP3 836
Affinity Capture-MS Homo sapiens
21 RAB11A 8766
Affinity Capture-MS Homo sapiens
22 BCL2L1 598
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
23 FLNB 2317
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
24 BCL2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
25 APH1A 51107
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 CTNNA1 1495
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 CSEN  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
28 UBQLN1 29979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
29 ICAM5 7087
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
30 NOTCH1 4851
Invivo Homo sapiens
31 DOCK3  
Invitro Homo sapiens
Two-hybrid Homo sapiens
32 HERPUD1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PSEN1 is involved
PathwayEvidenceSource
Degradation of the extracellular matrix TAS Reactome





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