Gene description for APH1B
Gene name APH1B gamma secretase subunit
Gene symbol APH1B
Other names/aliases APH-1B
PRO1328
PSFL
TAAV688
Species Homo sapiens
 Database cross references - APH1B
ExoCarta ExoCarta_83464
Vesiclepedia VP_83464
Entrez Gene 83464
HGNC 24080
MIM 607630
UniProt Q8WW43  
 APH1B identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for APH1B
Molecular Function
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
Biological Process
    protein processing GO:0016485 IDA
    Notch signaling pathway GO:0007219 TAS
    apoptotic signaling pathway GO:0097190 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    Notch receptor processing GO:0007220 TAS
    axon guidance GO:0007411 TAS
    positive regulation of catalytic activity GO:0043085 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    membrane protein intracellular domain proteolysis GO:0031293 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IDA
    transport vesicle GO:0030133 IDA
 Experiment description of studies that identified APH1B in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for APH1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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