Gene description for Ptpn12
Gene name protein tyrosine phosphatase, non-receptor type 12
Gene symbol Ptpn12
Other names/aliases P19-PTP
PTP-P19
PTP-PEST
PTPG1
Species Mus musculus
 Database cross references - Ptpn12
ExoCarta ExoCarta_19248
Vesiclepedia VP_19248
Entrez Gene 19248
UniProt P35831  
 Ptpn12 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ptpn12
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 ISO
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 ISO
    peptidyl-tyrosine dephosphorylation GO:0035335 ISO
    peptidyl-tyrosine dephosphorylation GO:0035335 ISS
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 IBA
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 ISO
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 ISS
    tissue regeneration GO:0042246 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 ISS
Subcellular Localization
    podosome GO:0002102 IDA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    focal adhesion GO:0005925 IEA
    cell projection GO:0042995 IEA
 Experiment description of studies that identified Ptpn12 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ptpn12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-RNA Mus musculus
2 SHC1 6464
Two-hybrid Homo sapiens
3 Shc1  
Affinity Capture-MS Mus musculus
4 Lpxn  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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