Gene description for Grap2
Gene name GRB2-related adaptor protein 2
Gene symbol Grap2
Other names/aliases GRAP-2
GRB2L
GRBLG
GRID
Gads
GrbX
Grf40
GrpL
Mona
P38
Species Mus musculus
 Database cross references - Grap2
ExoCarta ExoCarta_17444
Vesiclepedia VP_17444
Entrez Gene 17444
UniProt O89100  
 Grap2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Grap2
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    endosome GO:0005768 ISO
 Experiment description of studies that identified Grap2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Grap2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBL 867
Reconstituted Complex Homo sapiens
2 SOS1 6654
Reconstituted Complex Homo sapiens
3 sch  
Reconstituted Complex Mus musculus
4 KHDRBS1 10657
Reconstituted Complex Homo sapiens
5 Kit  
Affinity Capture-Western Mus musculus
6 ABL1 25
Reconstituted Complex Homo sapiens
7 EGFR 1956
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
9 Abl1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here