ExoCarta: Gene summary

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Gene description for FRS3
Gene name	fibroblast growth factor receptor substrate 3
Gene symbol	FRS3
Other names/aliases	FRS2-beta FRS2B FRS2beta SNT-2 SNT2
Species	Homo sapiens
Database cross references - FRS3
ExoCarta	ExoCarta_10817
Vesiclepedia	VP_10817
Entrez Gene	10817
HGNC	16970
MIM	607744
UniProt	O43559
FRS3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for FRS3

Molecular Function
	transmembrane receptor protein tyrosine kinase adaptor activity	GO:0005068	IBA
	fibroblast growth factor receptor binding	GO:0005104	IBA
	fibroblast growth factor receptor binding	GO:0005104	IPI
	protein binding	GO:0005515	IPI
	identical protein binding	GO:0042802	IPI
Biological Process
	signal transduction	GO:0007165	TAS
	fibroblast growth factor receptor signaling pathway	GO:0008543	IBA
	fibroblast growth factor receptor signaling pathway	GO:0008543	IGI
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	plasma membrane	GO:0005886	TAS

Experiment description of studies that identified FRS3 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for FRS3

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

GATA1

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRT76

51350

Two-hybrid

Homo sapiens

TCP10L

Two-hybrid

Homo sapiens

HOXD12

Two-hybrid

Homo sapiens

KRTAP11-1

Two-hybrid

Homo sapiens

ZNF438

Two-hybrid

Homo sapiens

FRS3

10817

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

ZNF552

Two-hybrid

Homo sapiens

AES

166

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

SPERT

Two-hybrid

Homo sapiens

CD2AP

23607

Affinity Capture-Western

Homo sapiens

MIIP

60672

Two-hybrid

Homo sapiens

ELAVL1

1994

Affinity Capture-RNA

Homo sapiens

C11orf1

Two-hybrid

Homo sapiens

NOV

4856

Two-hybrid

Homo sapiens

WDR83

Two-hybrid

Homo sapiens

COL8A1

1295

Two-hybrid

Homo sapiens

ANAPC11

Two-hybrid

Homo sapiens

KPRP

448834

Two-hybrid

Homo sapiens

CATSPER1

Two-hybrid

Homo sapiens

ERBB2

2064

Affinity Capture-Western

Homo sapiens

FGFR1

2260

Two-hybrid

Homo sapiens

ADAMTSL4

Two-hybrid

Homo sapiens

KRT34

Two-hybrid

Homo sapiens

POF1B

79983

Two-hybrid

Homo sapiens

TSGA10IP

Two-hybrid

Homo sapiens

ZNF124

Two-hybrid

Homo sapiens

PLSCR1

5359

Two-hybrid

Homo sapiens

TSPAN4

7106

Two-hybrid

Homo sapiens

RIMBP3C

Two-hybrid

Homo sapiens

LINGO1

84894

Two-hybrid

Homo sapiens

CCDC33

80125

Two-hybrid

Homo sapiens

SCNM1

Two-hybrid

Homo sapiens

CBL

867

Affinity Capture-Western

Homo sapiens

KRTAP6-2

Two-hybrid

Homo sapiens

SLAIN1

122060

Two-hybrid

Homo sapiens

CYSRT1

375791

Two-hybrid

Homo sapiens

GPRIN2

Two-hybrid

Homo sapiens

C19orf54

Two-hybrid

Homo sapiens

ECM1

1893

Two-hybrid

Homo sapiens

COPS3

8533

Two-hybrid

Homo sapiens

ID3

Two-hybrid

Homo sapiens

C22orf39

Two-hybrid

Homo sapiens

ID2

Two-hybrid

Homo sapiens

SLC22A18AS

Two-hybrid

Homo sapiens

NOTCH2NL

388677

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KATNBL1

Two-hybrid

Homo sapiens

C11orf45

Two-hybrid

Homo sapiens

KRT33B

3884

Two-hybrid

Homo sapiens

ANKRD55

Two-hybrid

Homo sapiens

IKZF3

Two-hybrid

Homo sapiens

KRT35

3886

Two-hybrid

Homo sapiens

KRTAP10-8

Two-hybrid

Homo sapiens

LOC100131303

Two-hybrid

Homo sapiens

XRCC6BP1

Two-hybrid

Homo sapiens

HOXA1

3198

Two-hybrid

Homo sapiens

PLLP

Two-hybrid

Homo sapiens

TGM7

Two-hybrid

Homo sapiens

ESM1

11082

Two-hybrid

Homo sapiens

NADSYN1

55191

Two-hybrid

Homo sapiens

ZMIZ2

Two-hybrid

Homo sapiens

BMPR2

659

Affinity Capture-MS

Homo sapiens

PLB1

Two-hybrid

Homo sapiens

RFX6

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

PPP1R32

Two-hybrid

Homo sapiens

CDPF1

Two-hybrid

Homo sapiens

KRTAP3-2

Two-hybrid

Homo sapiens

ZNF417

Two-hybrid

Homo sapiens

ZSCAN30

Two-hybrid

Homo sapiens

ISY1-RAB43

100534599

Two-hybrid

Homo sapiens

LRRC18

Two-hybrid

Homo sapiens

TRIP6

7205

Two-hybrid

Homo sapiens

KRTAP3-1

Two-hybrid

Homo sapiens

PDLIM7

9260

Two-hybrid

Homo sapiens

DCDC2B

Two-hybrid

Homo sapiens

STK16

8576

Two-hybrid

Homo sapiens

SPRY2

10253

Two-hybrid

Homo sapiens

WWOX

51741

Two-hybrid

Homo sapiens

BLZF1

Two-hybrid

Homo sapiens

NBPF19

Two-hybrid

Homo sapiens

PIH1D2

Two-hybrid

Homo sapiens

KRTAP1-1

Two-hybrid

Homo sapiens

ZNF69

Two-hybrid

Homo sapiens

TSC1

7248

Two-hybrid

Homo sapiens

CREB5

9586

Two-hybrid

Homo sapiens

TCF4

Two-hybrid

Homo sapiens

VGLL3

Two-hybrid

Homo sapiens

SPATA12

Two-hybrid

Homo sapiens

SH3KBP1

30011

Affinity Capture-Western

Homo sapiens

MBD3L1

Two-hybrid

Homo sapiens

INCA1

Two-hybrid

Homo sapiens

STH

Two-hybrid

Homo sapiens

KIAA0408

Two-hybrid

Homo sapiens

View the network

Pathways in which FRS3 is involved

Pathway	Evidence	Source
Activated NTRK2 signals through FRS2 and FRS3	IEA	Reactome
Disease	TAS	Reactome
Diseases of signal transduction by growth factor receptors and second messengers	TAS	Reactome
Downstream signaling of activated FGFR1	TAS	Reactome
Downstream signaling of activated FGFR2	TAS	Reactome
Downstream signaling of activated FGFR3	TAS	Reactome
Downstream signaling of activated FGFR4	TAS	Reactome
FRS-mediated FGFR1 signaling	TAS	Reactome
FRS-mediated FGFR2 signaling	TAS	Reactome
FRS-mediated FGFR3 signaling	TAS	Reactome
FRS-mediated FGFR4 signaling	TAS	Reactome
MAPK family signaling cascades	TAS	Reactome
MAPK1/MAPK3 signaling	TAS	Reactome
RAF/MAP kinase cascade	TAS	Reactome
RHO GTPase cycle	TAS	Reactome
RND1 GTPase cycle	TAS	Reactome
RND2 GTPase cycle	TAS	Reactome
Signal Transduction	IEA	Reactome
Signal Transduction	TAS	Reactome
Signaling by ALK fusions and activated point mutants	TAS	Reactome
Signaling by ALK in cancer	TAS	Reactome
Signaling by FGFR	TAS	Reactome
Signaling by FGFR1	TAS	Reactome
Signaling by FGFR2	TAS	Reactome
Signaling by FGFR3	TAS	Reactome
Signaling by FGFR4	TAS	Reactome
Signaling by NTRK2 (TRKB)	IEA	Reactome
Signaling by NTRKs	IEA	Reactome
Signaling by Receptor Tyrosine Kinases	IEA	Reactome
Signaling by Receptor Tyrosine Kinases	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here