Gene description for CUBN
Gene name cubilin (intrinsic factor-cobalamin receptor)
Gene symbol CUBN
Other names/aliases IFCR
MGA1
gp280
Species Homo sapiens
 Database cross references - CUBN
ExoCarta ExoCarta_8029
Entrez Gene 8029
HGNC 2548
MIM 602997
UniProt O60494  
 CUBN identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 21082674    
Urine 25452312    
 Gene ontology annotations for CUBN
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein homodimerization activity GO:0042803 IDA
    receptor activity GO:0004872 IEA
    transporter activity GO:0005215 TAS
    protein binding GO:0005515 IPI
    cobalamin binding GO:0031419 IEA
Biological Process
    cobalamin metabolic process GO:0009235 TAS
    small molecule metabolic process GO:0044281 TAS
    tissue homeostasis GO:0001894 NAS
    steroid metabolic process GO:0008202 TAS
    vitamin metabolic process GO:0006766 TAS
    vitamin D metabolic process GO:0042359 TAS
    water-soluble vitamin metabolic process GO:0006767 TAS
    cholesterol metabolic process GO:0008203 IEA
    lipoprotein metabolic process GO:0042157 TAS
    receptor-mediated endocytosis GO:0006898 NAS
    lipoprotein transport GO:0042953 IEA
    cobalamin transport GO:0015889 TAS
Subcellular Localization
    lysosomal lumen GO:0043202 TAS
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IEA
    endosome membrane GO:0010008 TAS
    extracellular exosome GO:0070062 IDA
    coated pit GO:0005905 IEA
    extrinsic component of external side of plasma membrane GO:0031232 NAS
    endocytic vesicle GO:0030139 IDA
    brush border membrane GO:0031526 NAS
    apical plasma membrane GO:0016324 IDA
    cytosol GO:0005829 TAS
    lysosomal membrane GO:0005765 IEA
    membrane GO:0016020 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified CUBN in exosomes
1
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 102
ISEV standards
IEM
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS.
Authors Zhang Y, Li Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
4
Experiment ID 238
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CUBN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRP2 4036
Invivo Homo sapiens
2 GIF  
Reconstituted Complex Homo sapiens
3 GC 2638
Affinity Capture-Western Homo sapiens
4 SCGB1A1  
Reconstituted Complex Homo sapiens
5 ALB 213
Invivo Homo sapiens
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