Gene description for PSTPIP1
Gene name proline-serine-threonine phosphatase interacting protein 1
Gene symbol PSTPIP1
Other names/aliases CD2BP1
CD2BP1L
CD2BP1S
H-PIP
PAPAS
PSTPIP
Species Homo sapiens
 Database cross references - PSTPIP1
ExoCarta ExoCarta_9051
Vesiclepedia VP_9051
Entrez Gene 9051
HGNC 9580
MIM 606347
UniProt O43586  
 PSTPIP1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PSTPIP1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    endocytosis GO:0006897 IEA
    inflammatory response GO:0006954 IEA
    cell adhesion GO:0007155 IEA
    signal transduction GO:0007165 TAS
    innate immune response GO:0045087 IEA
Subcellular Localization
    uropod GO:0001931 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    lamellipodium GO:0030027 IEA
    cleavage furrow GO:0032154 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PSTPIP1 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PSTPIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF580  
Two-hybrid Homo sapiens
2 ZNF175  
Two-hybrid Homo sapiens
3 MVP 9961
Two-hybrid Homo sapiens
4 TRAF3IP3  
Two-hybrid Homo sapiens
5 UBE2W  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 RPL34 6164
Affinity Capture-MS Homo sapiens
7 RPL32 6161
Affinity Capture-MS Homo sapiens
8 NCBP1 4686
Two-hybrid Homo sapiens
9 SDCBP 6386
Two-hybrid Homo sapiens
10 SHBG 6462
Two-hybrid Homo sapiens
11 RTP5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 MEFV  
Affinity Capture-Western Homo sapiens
13 WASL 8976
Reconstituted Complex Homo sapiens
14 WIPF1 7456
Affinity Capture-MS Homo sapiens
15 PYCARD 29108
Affinity Capture-MS Homo sapiens
16 DDX21 9188
Affinity Capture-MS Homo sapiens
17 BZW2 28969
Affinity Capture-MS Homo sapiens
18 CARS 833
Affinity Capture-MS Homo sapiens
19 EHHADH 1962
Two-hybrid Homo sapiens
20 ABL1 25
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
21 RPL36A 6173
Affinity Capture-MS Homo sapiens
22 TSGA10IP  
Two-hybrid Homo sapiens
23 RPL9 6133
Two-hybrid Homo sapiens
24 KANK2 25959
Two-hybrid Homo sapiens
25 LSM4 25804
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 RPL21 6144
Affinity Capture-MS Homo sapiens
27 RPL3 6122
Affinity Capture-MS Homo sapiens
28 RPL18A 6142
Affinity Capture-MS Homo sapiens
29 CWF19L2  
Two-hybrid Homo sapiens
30 MOS 4342
Two-hybrid Homo sapiens
31 FAM90A1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 ACTB 60
Affinity Capture-MS Homo sapiens
33 RPL35 11224
Affinity Capture-MS Homo sapiens
34 BUB3 9184
Two-hybrid Homo sapiens
35 IL16  
Two-hybrid Homo sapiens
36 HAPLN2  
Two-hybrid Homo sapiens
37 SPG7  
Two-hybrid Homo sapiens
38 FDFT1 2222
Affinity Capture-MS Homo sapiens
39 DHX40  
Two-hybrid Homo sapiens
40 WAS 7454
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
41 LIN37  
Two-hybrid Homo sapiens
42 PRR35  
Two-hybrid Homo sapiens
43 PCDHB14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 RUVBL1 8607
Affinity Capture-MS Homo sapiens
45 MCRS1  
Two-hybrid Homo sapiens
46 SUV420H2  
Affinity Capture-RNA Homo sapiens
47 ZNF408  
Two-hybrid Homo sapiens
48 TUBB 203068
Affinity Capture-MS Homo sapiens
49 PTPN12 5782
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 RPL31 6160
Affinity Capture-MS Homo sapiens
51 RNPS1 10921
Two-hybrid Homo sapiens
52 RPL35A 6165
Affinity Capture-MS Homo sapiens
53 FBXL18 80028
Two-hybrid Homo sapiens
54 HSPA1A 3303
Affinity Capture-MS Homo sapiens
55 CD2 914
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
56 RPL36 25873
Affinity Capture-MS Homo sapiens
57 RPL37A 6168
Affinity Capture-MS Homo sapiens
58 RPL23A 6147
Affinity Capture-MS Homo sapiens
59 ATIC 471
Affinity Capture-MS Homo sapiens
60 KHDRBS1 10657
Protein-peptide Homo sapiens
61 HHEX  
Two-hybrid Homo sapiens
62 DNM2 1785
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
63 HSF2BP  
Two-hybrid Homo sapiens
64 ZC2HC1C  
Two-hybrid Homo sapiens
65 PTPN18 26469
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
66 RPL26 6154
Affinity Capture-MS Homo sapiens
67 PRPF31 26121
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
68 SH2D4A 63898
Two-hybrid Homo sapiens
69 SCNM1  
Two-hybrid Homo sapiens
70 TPH1  
Two-hybrid Homo sapiens
71 TULP3 7289
Two-hybrid Homo sapiens
72 BZW1 9689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 MED28  
Affinity Capture-Western Homo sapiens
74 RPL29 6159
Affinity Capture-MS Homo sapiens
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