Gene description for ATIC
Gene name 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Gene symbol ATIC
Other names/aliases AICAR
AICARFT
HEL-S-70p
IMPCHASE
PURH
Species Homo sapiens
 Database cross references - ATIC
ExoCarta ExoCarta_471
Vesiclepedia VP_471
Entrez Gene 471
HGNC 794
MIM 601731
UniProt P31939  
 ATIC identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for ATIC
Molecular Function
    IMP cyclohydrolase activity GO:0003937 IBA
    IMP cyclohydrolase activity GO:0003937 IDA
    phosphoribosylaminoimidazolecarboxamide formyltransferase activity GO:0004643 IBA
    protein homodimerization activity GO:0042803 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    brainstem development GO:0003360 IEA
    nucleobase-containing compound metabolic process GO:0006139 TAS
    GMP biosynthetic process GO:0006177 IDA
    'de novo' IMP biosynthetic process GO:0006189 IBA
    'de novo' IMP biosynthetic process GO:0006189 IDA
    'de novo' IMP biosynthetic process GO:0006189 IEA
    cerebellum development GO:0021549 IEA
    cerebral cortex development GO:0021987 IEA
    animal organ regeneration GO:0031100 IEA
    'de novo' AMP biosynthetic process GO:0044208 IDA
    dihydrofolate metabolic process GO:0046452 IEA
    tetrahydrofolate biosynthetic process GO:0046654 IEA
    'de novo' XMP biosynthetic process GO:0097294 IDA
    cellular response to interleukin-7 GO:0098761 IEA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ATIC in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
16
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
35
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
37
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
44
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
45
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
46
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
47
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
48
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
49
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
50
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
51
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
52
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
53
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
54
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATIC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 LDHA 3939
Co-fractionation Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 OXCT1 5019
Co-fractionation Homo sapiens
5 ALDH16A1 126133
Co-fractionation Homo sapiens
6 OGFOD1  
Co-fractionation Homo sapiens
7 PTMA 5757
Co-fractionation Homo sapiens
8 RIT1 6016
Negative Genetic Homo sapiens
9 TARDBP 23435
Co-fractionation Homo sapiens
10 YWHAE 7531
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
11 CRK 1398
Co-fractionation Homo sapiens
12 UBA1 7317
Co-fractionation Homo sapiens
13 ECH1 1891
Co-fractionation Homo sapiens
14 RTN4 57142
Co-fractionation Homo sapiens
15 CMBL 134147
Affinity Capture-MS Homo sapiens
16 ARL3 403
Affinity Capture-MS Homo sapiens
17 PRC1 9055
Affinity Capture-MS Homo sapiens
18 STOM 2040
Affinity Capture-MS Homo sapiens
19 PGM1 5236
Co-fractionation Homo sapiens
20 MCTS1 28985
Co-fractionation Homo sapiens
21 YWHAG 7532
Co-fractionation Homo sapiens
22 ARMT1 79624
Co-fractionation Homo sapiens
23 CTH 1491
Co-fractionation Homo sapiens
24 IKBKE 9641
Affinity Capture-MS Homo sapiens
25 TATDN1 83940
Co-fractionation Homo sapiens
26 GART 2618
Co-fractionation Homo sapiens
27 NDUFAF1  
Affinity Capture-MS Homo sapiens
28 NME2 4831
Affinity Capture-MS Homo sapiens
29 NSFL1C 55968
Co-fractionation Homo sapiens
30 PRNP 5621
Two-hybrid Homo sapiens
31 ADSL 158
Co-fractionation Homo sapiens
32 AURKA 6790
Affinity Capture-MS Homo sapiens
33 NAE1 8883
Co-fractionation Homo sapiens
34 GMDS 2762
Co-fractionation Homo sapiens
35 TYMS 7298
Co-fractionation Homo sapiens
36 DIABLO 56616
Co-fractionation Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 CTBP1 1487
Co-fractionation Homo sapiens
39 SHMT1 6470
Co-fractionation Homo sapiens
40 DERA 51071
Co-fractionation Homo sapiens
41 CLNS1A 1207
Co-fractionation Homo sapiens
42 TRNT1 51095
Co-fractionation Homo sapiens
43 ACOT13  
Co-fractionation Homo sapiens
44 CDH1 999
Proximity Label-MS Homo sapiens
45 SREBF2 6721
Positive Genetic Homo sapiens
46 OGT 8473
Reconstituted Complex Homo sapiens
47 SPR 6697
Co-fractionation Homo sapiens
48 IFI16 3428
Affinity Capture-MS Homo sapiens
49 U2AF2 11338
Affinity Capture-MS Homo sapiens
50 SUMO2 6613
Co-fractionation Homo sapiens
51 FH 2271
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
52 PAICS 10606
Co-fractionation Homo sapiens
53 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
54 RBBP6 5930
Co-fractionation Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 IPO5 3843
Co-fractionation Homo sapiens
57 AHCY 191
Co-fractionation Homo sapiens
58 SAR1B 51128
Affinity Capture-MS Homo sapiens
59 SDSL 113675
Co-fractionation Homo sapiens
60 SIRT7  
Affinity Capture-MS Homo sapiens
61 CTNNB1 1499
Affinity Capture-MS Homo sapiens
62 ASB6 140459
Co-fractionation Homo sapiens
63 MYO1E 4643
Co-fractionation Homo sapiens
64 MTNR1A  
Two-hybrid Homo sapiens
65 EGFR 1956
PCA Homo sapiens
66 ELAC2 60528
Co-fractionation Homo sapiens
67 BAP1 8314
Negative Genetic Homo sapiens
68 RADIL  
Affinity Capture-MS Homo sapiens
69 GLO1 2739
Co-fractionation Homo sapiens
70 CDK2 1017
Affinity Capture-MS Homo sapiens
71 BAG1 573
Affinity Capture-MS Homo sapiens
72 JAK1 3716
Affinity Capture-MS Homo sapiens
73 KCNAB2 8514
Co-fractionation Homo sapiens
74 PLIN3 10226
Co-fractionation Homo sapiens
75 ISOC1 51015
Co-fractionation Homo sapiens
76 MSN 4478
Co-fractionation Homo sapiens
77 CEBPA  
Protein-peptide Homo sapiens
78 GNPDA1 10007
Co-fractionation Homo sapiens
79 PYHIN1  
Affinity Capture-MS Homo sapiens
80 PROSC 11212
Co-fractionation Homo sapiens
81 PAFAH1B1 5048
Co-fractionation Homo sapiens
82 FASN 2194
Positive Genetic Homo sapiens
83 SNRPA 6626
Affinity Capture-MS Homo sapiens
84 INSR 3643
Affinity Capture-Western Homo sapiens
85 HSPB1 3315
Co-fractionation Homo sapiens
86 CAPNS1 826
Co-fractionation Homo sapiens
87 STK26 51765
Co-fractionation Homo sapiens
88 DDX58 23586
Affinity Capture-RNA Homo sapiens
89 EEF2 1938
Co-fractionation Homo sapiens
90 ELF3 1999
Affinity Capture-MS Homo sapiens
91 TXN 7295
Co-fractionation Homo sapiens
92 DNM1L 10059
Co-fractionation Homo sapiens
93 ACTR2 10097
Affinity Capture-MS Homo sapiens
94 PSTPIP1 9051
Affinity Capture-MS Homo sapiens
95 PAPSS1 9061
Co-fractionation Homo sapiens
96 PINK1  
Affinity Capture-MS Homo sapiens
97 KCNAB1  
Co-fractionation Homo sapiens
98 CIT 11113
Affinity Capture-MS Homo sapiens
99 SERBP1 26135
Affinity Capture-MS Homo sapiens
100 CUL7 9820
Affinity Capture-MS Homo sapiens
101 KRAS 3845
Negative Genetic Homo sapiens
102 RPA3 6119
Proximity Label-MS Homo sapiens
103 BCAT1 586
Co-fractionation Homo sapiens
104 CAPN2 824
Co-fractionation Homo sapiens
View the network image/svg+xml



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