Gene description for SHMT1
Gene name serine hydroxymethyltransferase 1 (soluble)
Gene symbol SHMT1
Other names/aliases CSHMT
SHMT
Species Homo sapiens
 Database cross references - SHMT1
ExoCarta ExoCarta_6470
Entrez Gene 6470
HGNC 10850
MIM 182144
UniProt P34896  
 SHMT1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for SHMT1
Molecular Function
    protein homodimerization activity GO:0042803 IDA
    amino acid binding GO:0016597 IEA
    L-allo-threonine aldolase activity GO:0008732 IEA
    pyridoxal phosphate binding GO:0030170 IDA
    glycine hydroxymethyltransferase activity GO:0004372 TAS
    protein binding GO:0005515 IPI
Biological Process
    protein homotetramerization GO:0051289 IEA
    water-soluble vitamin metabolic process GO:0006767 TAS
    carnitine biosynthetic process GO:0045329 TAS
    small molecule metabolic process GO:0044281 TAS
    glycine biosynthetic process from serine GO:0019264 IEA
    tetrahydrofolate interconversion GO:0035999 IEA
    vitamin metabolic process GO:0006766 TAS
    folic acid metabolic process GO:0046655 TAS
    purine nucleobase biosynthetic process GO:0009113 IDA
    cellular nitrogen compound metabolic process GO:0034641 TAS
    protein tetramerization GO:0051262 IDA
    L-serine catabolic process GO:0006565 IDA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 IEA
 Experiment description of studies that identified SHMT1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SHMT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CSNK2B 1460
Invitro Homo sapiens
2 PIM1  
Two-hybrid Homo sapiens
3 PRSS23 11098
Two-hybrid Homo sapiens
4 DLEU1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SHMT1 is involved
PathwayEvidenceSource
Carnitine synthesis TAS Reactome
Metabolism of folate and pterines TAS Reactome





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