Gene description for IFT122
Gene name intraflagellar transport 122
Gene symbol IFT122
Other names/aliases CED
CED1
SPG
WDR10
WDR10p
WDR140
Species Homo sapiens
 Database cross references - IFT122
ExoCarta ExoCarta_55764
Vesiclepedia VP_55764
Entrez Gene 55764
HGNC 13556
MIM 606045
UniProt Q9HBG6  
 IFT122 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT122
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    neural tube closure GO:0001843 ISS
    embryonic body morphogenesis GO:0010172 ISS
    spinal cord dorsal/ventral patterning GO:0021513 ISS
    embryonic heart tube development GO:0035050 ISS
    embryonic forelimb morphogenesis GO:0035115 ISS
    intracellular signal transduction GO:0035556 ISS
    intraciliary anterograde transport GO:0035720 ISS
    intraciliary retrograde transport GO:0035721 IBA
    intraciliary retrograde transport GO:0035721 ISS
    intraciliary retrograde transport GO:0035721 NAS
    intraciliary transport GO:0042073 IMP
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    camera-type eye morphogenesis GO:0048593 ISS
    limb development GO:0060173 ISS
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 ISS
    cilium assembly GO:0060271 NAS
    embryonic heart tube left/right pattern formation GO:0060971 ISS
    protein localization to cilium GO:0061512 IBA
    protein localization to cilium GO:0061512 IMP
    protein localization to cilium GO:0061512 IMP
    establishment of protein localization to organelle GO:0072594 ISS
    non-motile cilium assembly GO:1905515 IBA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cilium GO:0005929 IDA
    cilium GO:0005929 ISS
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    membrane GO:0016020 HDA
    intraciliary transport particle A GO:0030991 IBA
    intraciliary transport particle A GO:0030991 IDA
    intraciliary transport particle A GO:0030991 IPI
    photoreceptor connecting cilium GO:0032391 ISS
    ciliary basal body GO:0036064 IDA
    ciliary tip GO:0097542 TAS
    non-motile cilium GO:0097730 IBA
 Experiment description of studies that identified IFT122 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT122
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PFKP 5214
Affinity Capture-MS Homo sapiens
3 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
4 NTRK1 4914
Affinity Capture-MS Homo sapiens
5 NPAS1  
Affinity Capture-MS Homo sapiens
6 PPM1M  
Affinity Capture-MS Homo sapiens
7 FBXW5  
Affinity Capture-MS Homo sapiens
8 IFT140 9742
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CCDC40  
Affinity Capture-MS Homo sapiens
10 CIB2  
Affinity Capture-MS Homo sapiens
11 BAG2 9532
Affinity Capture-MS Homo sapiens
12 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 BAG5 9529
Affinity Capture-MS Homo sapiens
14 WDR35 57539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FAM96A  
Affinity Capture-MS Homo sapiens
17 GFOD1  
Affinity Capture-MS Homo sapiens
18 HDHD1  
Affinity Capture-MS Homo sapiens
19 Vangl1  
Affinity Capture-MS Mus musculus
20 PDDC1 347862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TFPT  
Affinity Capture-MS Homo sapiens
22 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 METTL21B  
Affinity Capture-MS Homo sapiens
24 B4GALT2  
Affinity Capture-MS Homo sapiens
25 C11orf74  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NEK7 140609
Affinity Capture-MS Homo sapiens
27 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
28 C12orf74  
Affinity Capture-MS Homo sapiens
29 IFI35 3430
Affinity Capture-MS Homo sapiens
30 LOXL4 84171
Affinity Capture-MS Homo sapiens
31 INF2 64423
Affinity Capture-MS Homo sapiens
32 SOHLH1  
Affinity Capture-MS Homo sapiens
33 TUBB 203068
Affinity Capture-MS Homo sapiens
34 IARS2 55699
Affinity Capture-MS Homo sapiens
35 D2HGDH 728294
Affinity Capture-MS Homo sapiens
36 IFT43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PPARD  
Affinity Capture-MS Homo sapiens
38 DNAJA1 3301
Affinity Capture-MS Homo sapiens
39 HNRNPAB 3182
Cross-Linking-MS (XL-MS) Homo sapiens
40 PIH1D1  
Affinity Capture-MS Homo sapiens
41 PTGES3 10728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 FAM167A  
Affinity Capture-MS Homo sapiens
43 TULP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RNF157 114804
Affinity Capture-MS Homo sapiens
45 WDR19 57728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CDC7  
Affinity Capture-MS Homo sapiens
47 SDF4 51150
Affinity Capture-MS Homo sapiens
48 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 NDUFAF3 25915
Affinity Capture-MS Homo sapiens
50 HSPB8 26353
Affinity Capture-MS Homo sapiens
51 TTC21B 79809
Affinity Capture-MS Homo sapiens
52 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 UBXN6 80700
Affinity Capture-MS Homo sapiens
54 SULT1C4  
Affinity Capture-MS Homo sapiens
55 ATG7 10533
Affinity Capture-MS Homo sapiens
56 KLHL14 57565
Affinity Capture-MS Homo sapiens
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