Gene description for PRDM16
Gene name PR domain containing 16
Gene symbol PRDM16
Other names/aliases CMD1LL
LVNC8
MEL1
PFM13
Species Homo sapiens
 Database cross references - PRDM16
ExoCarta ExoCarta_63976
Vesiclepedia VP_63976
Entrez Gene 63976
HGNC 14000
MIM 605557
UniProt Q9HAZ2  
 PRDM16 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for PRDM16
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IBA
    transcription coregulator activity GO:0003712 IBA
    transcription coactivator activity GO:0003713 ISS
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
    metal ion binding GO:0046872 IEA
    histone H3K9 methyltransferase activity GO:0046974 ISS
    DNA-binding transcription factor binding GO:0140297 IPI
    histone H3 methyltransferase activity GO:0140938 TAS
    histone H3K9me2 methyltransferase activity GO:0140947 IEA
    histone H3K9 monomethyltransferase activity GO:0140948 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IMP
    negative regulation of granulocyte differentiation GO:0030853 IDA
    methylation GO:0032259 IEA
    regulation of cellular respiration GO:0043457 ISS
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    brown fat cell differentiation GO:0050873 ISS
    heterochromatin organization GO:0070828 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    aggresome GO:0016235 IDA
    transcription repressor complex GO:0017053 ISS
 Experiment description of studies that identified PRDM16 in exosomes
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for PRDM16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX6 1656
Two-hybrid Homo sapiens
2 SOX7  
Proximity Label-MS Homo sapiens
3 RCOR2 283248
Affinity Capture-MS Homo sapiens
4 CUL3 8452
Affinity Capture-MS Homo sapiens
5 BTBD17  
Affinity Capture-MS Homo sapiens
6 RPS26 6231
Affinity Capture-MS Homo sapiens
7 SOX6  
Proximity Label-MS Homo sapiens
8 NEK6 10783
Two-hybrid Homo sapiens
9 SKI 6497
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 CTBP2 1488
Affinity Capture-MS Homo sapiens
11 ELK3  
Proximity Label-MS Homo sapiens
12 POLR3C 10623
Two-hybrid Homo sapiens
13 SMAD3 4088
Affinity Capture-Western Homo sapiens
14 LONRF1  
Two-hybrid Homo sapiens
15 GLMN 11146
Two-hybrid Homo sapiens
16 PRSS3 5646
Affinity Capture-MS Homo sapiens
17 KLF3  
Proximity Label-MS Homo sapiens
18 GAPDHS 26330
Affinity Capture-MS Homo sapiens
19 PHIP 55023
Affinity Capture-MS Homo sapiens
20 TFAP4 7023
Affinity Capture-MS Homo sapiens
21 MIPOL1  
Two-hybrid Homo sapiens
22 CTBP1 1487
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 XPA  
Two-hybrid Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 S100A14 57402
Affinity Capture-MS Homo sapiens
26 RCOR1  
Affinity Capture-MS Homo sapiens
27 SMAD2 4087
Affinity Capture-Western Homo sapiens
28 LENG8  
Two-hybrid Homo sapiens
29 TUBA1C 84790
Affinity Capture-MS Homo sapiens
30 RPA3 6119
Proximity Label-MS Homo sapiens
31 ARRB1 408
Two-hybrid Homo sapiens
32 TCHP  
Two-hybrid Homo sapiens
33 CCDC13 152206
Two-hybrid Homo sapiens
34 PLK4  
Two-hybrid Homo sapiens
35 Ctbp2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
36 XRCC6 2547
Proximity Label-MS Homo sapiens
37 DLGAP4 22839
Affinity Capture-MS Homo sapiens
38 HOXB2  
Proximity Label-MS Homo sapiens
39 TEX10 54881
Two-hybrid Homo sapiens
40 ACOT8 10005
Two-hybrid Homo sapiens
41 KLF12  
Proximity Label-MS Homo sapiens
42 SPDEF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 C6orf165  
Two-hybrid Homo sapiens
44 ZNF516 9658
Affinity Capture-MS Homo sapiens
45 DMBT1 1755
Affinity Capture-MS Homo sapiens
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