Gene description for ZNF516
Gene name zinc finger protein 516
Gene symbol ZNF516
Other names/aliases HsT287
Species Homo sapiens
 Database cross references - ZNF516
ExoCarta ExoCarta_9658
Entrez Gene 9658
HGNC 28990
MIM 615114
UniProt Q92618  
 ZNF516 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ZNF516
Molecular Function
    core promoter proximal region sequence-specific DNA binding GO:0000987 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    brown fat cell differentiation GO:0050873 ISS
    adipose tissue development GO:0060612 ISS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
 Experiment description of studies that identified ZNF516 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ZNF516
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ZNF516 is involved
No pathways found





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