Gene description for ZNF516
Gene name zinc finger protein 516
Gene symbol ZNF516
Other names/aliases HsT287
Species Homo sapiens
 Database cross references - ZNF516
ExoCarta ExoCarta_9658
Vesiclepedia VP_9658
Entrez Gene 9658
HGNC 28990
MIM 615114
UniProt Q92618  
 ZNF516 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ZNF516
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    cis-regulatory region sequence-specific DNA binding GO:0000987 ISS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    DNA-binding transcription factor binding GO:0140297 IEA
Biological Process
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    response to cold GO:0009409 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISS
    brown fat cell differentiation GO:0050873 ISS
    adipose tissue development GO:0060612 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
 Experiment description of studies that identified ZNF516 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ZNF516
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 TLX1  
Proximity Label-MS Homo sapiens
3 PAX6  
Proximity Label-MS Homo sapiens
4 ASF1A 25842
Proximity Label-MS Homo sapiens
5 CSNK2B 1460
Affinity Capture-MS Homo sapiens
6 LHX6  
Proximity Label-MS Homo sapiens
7 DCAF7 10238
Proximity Label-MS Homo sapiens
8 DYRK1B  
Affinity Capture-MS Homo sapiens
9 CTBP2 1488
Affinity Capture-MS Homo sapiens
10 ZEB1  
Affinity Capture-MS Homo sapiens
11 LCOR 84458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RPA2 6118
Proximity Label-MS Homo sapiens
13 GATA2  
Proximity Label-MS Homo sapiens
14 KLF3  
Proximity Label-MS Homo sapiens
15 HYPK 25764
Affinity Capture-MS Homo sapiens
16 NR3C1 2908
Proximity Label-MS Homo sapiens
17 ZBP1  
Affinity Capture-MS Homo sapiens
18 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CTBP1 1487
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
20 TEAD1  
Proximity Label-MS Homo sapiens
21 HIST3H3 8290
Biochemical Activity Homo sapiens
22 KLF12  
Proximity Label-MS Homo sapiens
23 APEX1 328
Proximity Label-MS Homo sapiens
24 KDM1A 23028
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
25 PRDM16 63976
Affinity Capture-MS Homo sapiens
26 KLF8  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 EGFR 1956
Affinity Capture-MS Homo sapiens
28 HDAC2 3066
Affinity Capture-MS Homo sapiens
29 S100B 6285
Affinity Capture-MS Homo sapiens
30 PML 5371
Affinity Capture-MS Homo sapiens
31 NOL4  
Affinity Capture-MS Homo sapiens
32 KLF15  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
33 ZBTB42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SP7  
Proximity Label-MS Homo sapiens
35 ZBTB18  
Affinity Capture-MS Homo sapiens
36 RCOR1  
Affinity Capture-MS Homo sapiens
37 CEBPA  
Proximity Label-MS Homo sapiens
38 SFMBT1 51460
Affinity Capture-MS Homo sapiens
39 VSX1  
Proximity Label-MS Homo sapiens
40 KLF4  
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ZNF516 is involved
No pathways found





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