Gene description for ARHGAP33
Gene name Rho GTPase activating protein 33
Gene symbol ARHGAP33
Other names/aliases NOMA-GAP
SNX26
TCGAP
Species Homo sapiens
 Database cross references - ARHGAP33
ExoCarta ExoCarta_115703
Vesiclepedia VP_115703
Entrez Gene 115703
HGNC 23085
MIM 614902
UniProt O14559  
 ARHGAP33 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ARHGAP33
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IBA
    response to toxic substance GO:0009636 IEA
    protein transport GO:0015031 IEA
    regulation of dendritic spine morphogenesis GO:0061001 IEA
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    fibrillar center GO:0001650 IBA
    nucleoplasm GO:0005654 IBA
    Golgi apparatus GO:0005794 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    cell cortex GO:0005938 IBA
    postsynaptic density GO:0014069 IEA
    actin cytoskeleton GO:0015629 IBA
    protein-containing complex GO:0032991 IDA
    dendritic spine GO:0043197 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified ARHGAP33 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP33
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRC1 9055
Affinity Capture-MS Homo sapiens
2 MECP2 4204
Affinity Capture-MS Homo sapiens
3 RAC1 5879
Two-hybrid Homo sapiens
4 SMARCA2 6595
Affinity Capture-MS Homo sapiens
5 TP53 7157
Affinity Capture-MS Homo sapiens
6 NEK6 10783
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
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