Gene description for ARHGAP33
Gene name Rho GTPase activating protein 33
Gene symbol ARHGAP33
Other names/aliases NOMA-GAP
SNX26
TCGAP
Species Homo sapiens
 Database cross references - ARHGAP33
ExoCarta ExoCarta_115703
Entrez Gene 115703
HGNC 23085
MIM 614902
UniProt O14559  
 ARHGAP33 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ARHGAP33
Molecular Function
    phosphatidylinositol binding GO:0035091 IEA
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
Biological Process
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    protein transport GO:0015031 IEA
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified ARHGAP33 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP33
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ARHGAP33 is involved
PathwayEvidenceSource
Rho GTPase cycle TAS Reactome





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