Experiment description of studies that identified CAPS in exosomes

1 Experiment ID 34 ISEV standards ✔ EM|IEM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD63|MHCI|MHCII EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19190083     Organism Homo sapiens Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense. Authors Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK Journal name FASEB Publication year 2009 Sample Tracheobronchial cells Sample name Tracheobronchial epithelial cells Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density 1.16 -1.18 g/mL Molecules identified in the study Protein mRNA Methods used in the study Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS

2 Experiment ID 196 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix|TSG101|HSP70 EV Cytosolic markers ✔ CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G. Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal high density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 197 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix|TSG101|HSP70 EV Cytosolic markers ✔ CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G. Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal low density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for CAPS

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which CAPS is involved

No pathways found