Gene description for OTUD3
Gene name OTU deubiquitinase 3
Gene symbol OTUD3
Other names/aliases DUBA4
Species Homo sapiens
 Database cross references - OTUD3
ExoCarta ExoCarta_23252
Vesiclepedia VP_23252
Entrez Gene 23252
HGNC 29038
MIM 611758
UniProt Q5T2D3  
 OTUD3 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for OTUD3
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 IMP
    cysteine-type deubiquitinase activity GO:0004843 TAS
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 IDA
    protein deubiquitination GO:0016579 TAS
    cellular response to nutrient levels GO:0031669 IDA
    protein K11-linked deubiquitination GO:0035871 IDA
    protein K11-linked deubiquitination GO:0035871 IDA
    protein K6-linked deubiquitination GO:0044313 IDA
    protein K6-linked deubiquitination GO:0044313 IDA
    protein stabilization GO:0050821 IBA
    protein stabilization GO:0050821 IDA
    protein stabilization GO:0050821 IDA
    protein stabilization GO:0050821 IMP
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IDA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IMP
    protein K48-linked deubiquitination GO:0071108 IDA
    protein K48-linked deubiquitination GO:0071108 IDA
    protein K27-linked deubiquitination GO:1990167 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified OTUD3 in exosomes
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for OTUD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YBX1 4904
Affinity Capture-MS Homo sapiens
2 KPTN  
Affinity Capture-MS Homo sapiens
3 HSPA5 3309
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 PLS1 5357
Affinity Capture-MS Homo sapiens
5 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 ZFP36  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 MTA2 9219
Affinity Capture-MS Homo sapiens
8 DGKA 1606
Cross-Linking-MS (XL-MS) Homo sapiens
9 IREB2 3658
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 ITFG2  
Affinity Capture-MS Homo sapiens
11 AURKA 6790
Affinity Capture-Western Homo sapiens
12 ADAP1  
Cross-Linking-MS (XL-MS) Homo sapiens
13 IRS4 8471
Affinity Capture-MS Homo sapiens
14 MIB1 57534
Affinity Capture-MS Homo sapiens
15 HRAS 3265
Affinity Capture-MS Homo sapiens
16 PTEN 5728
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
17 SQSTM1 8878
Proximity Label-MS Homo sapiens
18 SRSF5 6430
Affinity Capture-MS Homo sapiens
19 UBC 7316
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
20 KBTBD7  
Affinity Capture-MS Homo sapiens
21 TRIM25 7706
Affinity Capture-MS Homo sapiens
22 OTUD3 23252
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 MLF1  
Affinity Capture-MS Homo sapiens
24 GNA11 2767
Affinity Capture-MS Homo sapiens
25 OTUD4  
Affinity Capture-MS Homo sapiens
26 NCL 4691
Affinity Capture-MS Homo sapiens
27 MYH9 4627
Affinity Capture-MS Homo sapiens
28 GNB2 2783
Affinity Capture-MS Homo sapiens
29 GNAT3 346562
Affinity Capture-MS Homo sapiens
30 UBE2L3 7332
Biochemical Activity Homo sapiens
31 ACTN4 81
Affinity Capture-Western Homo sapiens
32 GNG10 2790
Affinity Capture-MS Homo sapiens
33 MB21D1  
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
34 GNA13 10672
Affinity Capture-MS Homo sapiens
35 DHX9 1660
Affinity Capture-MS Homo sapiens
36 INTS12  
Cross-Linking-MS (XL-MS) Homo sapiens
37 RP2 6102
Affinity Capture-MS Homo sapiens
38 ZNF268  
Affinity Capture-MS Homo sapiens
39 KPRP 448834
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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