Gene description for GNAT3
Gene name guanine nucleotide binding protein, alpha transducing 3
Gene symbol GNAT3
Other names/aliases GDCA
Species Homo sapiens
 Database cross references - GNAT3
ExoCarta ExoCarta_346562
Vesiclepedia VP_346562
Entrez Gene 346562
HGNC 22800
MIM 139395
UniProt A8MTJ3  
 GNAT3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GNAT3
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 IBA
    GTP binding GO:0005525 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    phototransduction, visible light GO:0007603 IBA
    response to nicotine GO:0035094 IEA
    detection of light stimulus involved in visual perception GO:0050908 IBA
    sensory perception of sweet taste GO:0050916 IBA
    sensory perception of umami taste GO:0050917 IEA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    cytoplasm GO:0005737 IBA
    heterotrimeric G-protein complex GO:0005834 IBA
    plasma membrane GO:0005886 TAS
    axoneme GO:0005930 IEA
    apical plasma membrane GO:0016324 IEA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified GNAT3 in sEVs
1
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNAT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNG13  
Reconstituted Complex Homo sapiens
2 Haus1  
Affinity Capture-MS Mus musculus
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 RAB5C 5878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SNTA1 6640
Affinity Capture-MS Homo sapiens
6 KIF20A 10112
Affinity Capture-MS Homo sapiens
7 CHMP4B 128866
Affinity Capture-MS Homo sapiens
8 BKRF1  
Affinity Capture-MS
9 OTUD3 23252
Affinity Capture-MS Homo sapiens
10 KIF23 9493
Affinity Capture-MS Homo sapiens
11 KIF14 9928
Affinity Capture-MS Homo sapiens
12 ECT2 1894
Affinity Capture-MS Homo sapiens
13 GNAI1 2770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GNAT3 is involved
PathwayEvidenceSource
Activation of GABAB receptors IEA Reactome
Activation of GABAB receptors TAS Reactome
Adenylate cyclase inhibitory pathway IEA Reactome
Adenylate cyclase inhibitory pathway TAS Reactome
ADORA2B mediated anti-inflammatory cytokines production IEA Reactome
ADP signalling through P2Y purinoceptor 12 TAS Reactome
Anti-inflammatory response favouring Leishmania parasite infection IEA Reactome
Disease IEA Reactome
ESR-mediated signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
G alpha (i) signalling events TAS Reactome
G alpha (i) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G alpha (z) signalling events IEA Reactome
G-protein mediated events IEA Reactome
G-protein mediated events TAS Reactome
GABA B receptor activation IEA Reactome
GABA B receptor activation TAS Reactome
GABA receptor activation IEA Reactome
GABA receptor activation TAS Reactome
GPCR downstream signalling TAS Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
Hemostasis TAS Reactome
Incretin synthesis, secretion, and inactivation TAS Reactome
Infectious disease IEA Reactome
Leishmania infection IEA Reactome
Leishmania parasite growth and survival IEA Reactome
Metabolism of proteins TAS Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Opioid Signalling IEA Reactome
Opioid Signalling TAS Reactome
Parasitic Infection Pathways IEA Reactome
Peptide hormone metabolism TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Sensory Perception IEA Reactome
Sensory Perception TAS Reactome
Sensory perception of sweet, bitter, and umami (glutamate) taste IEA Reactome
Sensory perception of sweet, bitter, and umami (glutamate) taste TAS Reactome
Sensory perception of taste IEA Reactome
Sensory perception of taste TAS Reactome
Signal amplification TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by Nuclear Receptors TAS Reactome
Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) TAS Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome





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