Gene description for GNAT3
Gene name guanine nucleotide binding protein, alpha transducing 3
Gene symbol GNAT3
Other names/aliases GDCA
Species Homo sapiens
 Database cross references - GNAT3
ExoCarta ExoCarta_346562
Entrez Gene 346562
HGNC 22800
MIM 139395
UniProt A8MTJ3  
 GNAT3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GNAT3
Molecular Function
    metal ion binding GO:0046872 IEA
    GTP binding GO:0005525 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    signal transducer activity GO:0004871 IBA
    GTPase activity GO:0003924 IBA
    G-protein coupled receptor binding GO:0001664 IBA
Biological Process
    response to nicotine GO:0035094 IEA
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    platelet activation GO:0030168 TAS
    sensory perception of sweet taste GO:0050916 IBA
    blood coagulation GO:0007596 TAS
    synaptic transmission GO:0007268 TAS
    sensory perception of umami taste GO:0050917 IEA
    adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway GO:0007193 TAS
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IBA
    metabolic process GO:0008152 IEA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    apical plasma membrane GO:0016324 IEA
    heterotrimeric G-protein complex GO:0005834 IBA
    photoreceptor outer segment GO:0001750 IBA
    photoreceptor inner segment GO:0001917 IBA
    axoneme GO:0005930 IEA
    protein complex GO:0043234 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified GNAT3 in exosomes
1
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNAT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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