Gene description for OSBPL3
Gene name oxysterol binding protein-like 3
Gene symbol OSBPL3
Other names/aliases ORP-3
ORP3
OSBP3
Species Homo sapiens
 Database cross references - OSBPL3
ExoCarta ExoCarta_26031
Vesiclepedia VP_26031
Entrez Gene 26031
HGNC 16370
MIM 606732
UniProt Q9H4L5  
 OSBPL3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for OSBPL3
Molecular Function
    protein binding GO:0005515 IPI
    cholesterol binding GO:0015485 IBA
    cholesterol binding GO:0015485 IDA
    sterol transfer activity GO:0120015 TAS
Biological Process
    bile acid biosynthetic process GO:0006699 TAS
    sterol transport GO:0015918 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 IBA
    nuclear membrane GO:0031965 IDA
    filopodium tip GO:0032433 IEA
    perinuclear endoplasmic reticulum GO:0097038 IBA
    perinuclear endoplasmic reticulum GO:0097038 IDA
 Experiment description of studies that identified OSBPL3 in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for OSBPL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 NID2 22795
Affinity Capture-MS Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 FGF11  
Affinity Capture-MS Homo sapiens
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 MMGT1 93380
Affinity Capture-MS Homo sapiens
9 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ABT1 29777
Affinity Capture-MS Homo sapiens
11 PITPNM3 83394
Affinity Capture-MS Homo sapiens
12 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ZFPL1 7542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ERH 2079
Affinity Capture-MS Homo sapiens
18 S100A6 6277
Affinity Capture-MS Homo sapiens
19 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KIAA1324 57535
Affinity Capture-MS Homo sapiens
21 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 EMID1  
Affinity Capture-MS Homo sapiens
23 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
24 THAP3  
Affinity Capture-MS Homo sapiens
25 ARRDC1 92714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 KLRD1  
Affinity Capture-MS Homo sapiens
28 HOOK3 84376
Proximity Label-MS Homo sapiens
29 Tmed2 56334
Affinity Capture-MS Mus musculus
30 TSPYL1 7259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HDAC11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 METTL21B  
Affinity Capture-MS Homo sapiens
33 Ktn1  
Affinity Capture-MS Mus musculus
34 ARMC8 25852
Affinity Capture-MS Homo sapiens
35 ILVBL 10994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 CBY1  
Affinity Capture-MS Homo sapiens
38 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PHTF2  
Affinity Capture-MS Homo sapiens
40 TFPT  
Affinity Capture-MS Homo sapiens
41 TTI2 80185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 BTF3 689
Affinity Capture-MS Homo sapiens
43 ACTR3B 57180
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 FGF13  
Affinity Capture-MS Homo sapiens
45 SP110  
Affinity Capture-MS Homo sapiens
46 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PTPN1 5770
Proximity Label-MS Homo sapiens
48 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RNPS1 10921
Affinity Capture-MS Homo sapiens
50 YWHAQ 10971
Affinity Capture-MS Homo sapiens
51 Junb  
Affinity Capture-MS Mus musculus
52 ENO1 2023
Affinity Capture-RNA Homo sapiens
53 SMC1A 8243
Affinity Capture-MS Homo sapiens
54 RHOB 388
Proximity Label-MS Homo sapiens
55 Rpl35 66489
Affinity Capture-MS Mus musculus
56 ADCYAP1  
Affinity Capture-MS Homo sapiens
57 KCTD3  
Affinity Capture-MS Homo sapiens
58 RPL36AL 6166
Affinity Capture-MS Homo sapiens
59 KRAS 3845
Proximity Label-MS Homo sapiens
60 PPP2R5B 5526
Affinity Capture-MS Homo sapiens
61 GLTSCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 OSBPL7  
Affinity Capture-MS Homo sapiens
63 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TRAF1 7185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here