Gene description for PPP2R5B
Gene name protein phosphatase 2, regulatory subunit B', beta
Gene symbol PPP2R5B
Other names/aliases B56B
PR61B
Species Homo sapiens
 Database cross references - PPP2R5B
ExoCarta ExoCarta_5526
Vesiclepedia VP_5526
Entrez Gene 5526
HGNC 9310
MIM 601644
UniProt Q15173  
 PPP2R5B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPP2R5B
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 TAS
    protein phosphatase activator activity GO:0072542 IBA
Biological Process
    signal transduction GO:0007165 IEA
    positive regulation of neuron projection development GO:0010976 IEA
    positive regulation of protein-containing complex assembly GO:0031334 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of neurotrophin TRK receptor signaling pathway GO:0051388 IEA
    negative regulation of G0 to G1 transition GO:0070317 IEA
    cellular response to growth factor stimulus GO:0071363 IEA
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IBA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PPP2R5B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPP2R5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DUSP19  
Affinity Capture-MS Homo sapiens
2 KNDC1  
Affinity Capture-MS Homo sapiens
3 CDCA2 157313
Affinity Capture-MS Homo sapiens
4 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
5 WNT3A 89780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 USP15 9958
Affinity Capture-MS Homo sapiens
7 MAPK1 5594
Biochemical Activity Homo sapiens
8 FAM43A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SGOL1  
Affinity Capture-MS Homo sapiens
10 CHEK2  
Reconstituted Complex Homo sapiens
11 C1orf21  
Affinity Capture-MS Homo sapiens
12 IER3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 PSMB8 5696
Co-fractionation Homo sapiens
14 ERGIC2 51290
Affinity Capture-MS Homo sapiens
15 OSBPL3 26031
Affinity Capture-MS Homo sapiens
16 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
17 CCNG2  
Affinity Capture-Western Homo sapiens
18 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
19 USP19 10869
Affinity Capture-MS Homo sapiens
20 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
21 GRIN2A  
Affinity Capture-MS Homo sapiens
22 SOGA3  
Affinity Capture-MS Homo sapiens
23 NHSL1  
Affinity Capture-MS Homo sapiens
24 PPP2CB 5516
Affinity Capture-MS Homo sapiens
25 AIM1 202
Affinity Capture-MS Homo sapiens
26 MVB12A 93343
Affinity Capture-MS Homo sapiens
27 KLHL14 57565
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP2R5B is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Costimulation by the CD28 family TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
CTNNB1 S33 mutants aren't phosphorylated TAS Reactome
CTNNB1 S37 mutants aren't phosphorylated TAS Reactome
CTNNB1 S45 mutants aren't phosphorylated TAS Reactome
CTNNB1 T41 mutants aren't phosphorylated TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRE1alpha activates chaperones TAS Reactome
M Phase TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet homeostasis TAS Reactome
Platelet sensitization by LDL TAS Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by AMER1 mutants TAS Reactome
Signaling by APC mutants TAS Reactome
Signaling by AXIN mutants TAS Reactome
Signaling by CTNNB1 phospho-site mutants TAS Reactome
Signaling by GSK3beta mutants TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Signaling by WNT in cancer TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCF dependent signaling in response to WNT IEA Reactome
Truncations of AMER1 destabilize the destruction complex TAS Reactome
Unfolded Protein Response (UPR) TAS Reactome
XBP1(S) activates chaperone genes TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here