Gene description for GRK6
Gene name G protein-coupled receptor kinase 6
Gene symbol GRK6
Other names/aliases GPRK6
Species Homo sapiens
 Database cross references - GRK6
ExoCarta ExoCarta_2870
Vesiclepedia VP_2870
Entrez Gene 2870
HGNC 4545
MIM 600869
UniProt P43250  
 GRK6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GRK6
Molecular Function
    G protein-coupled receptor kinase activity GO:0004703 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    beta-adrenergic receptor kinase activity GO:0047696 IDA
    beta-adrenergic receptor kinase activity GO:0047696 IMP
    beta-adrenergic receptor kinase activity GO:0047696 TAS
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    regulation of G protein-coupled receptor signaling pathway GO:0008277 TAS
    regulation of signal transduction GO:0009966 IBA
    Wnt signaling pathway GO:0016055 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified GRK6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GRK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 TBXA2R 6915
Biochemical Activity Homo sapiens
3 ENDOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 EIF1AD 84285
Affinity Capture-MS Homo sapiens
5 SNCA 6622
Biochemical Activity Homo sapiens
6 SNCB 6620
Biochemical Activity Homo sapiens
7 ITGA5 3678
Two-hybrid Homo sapiens
8 RCVRN  
Affinity Capture-Western Homo sapiens
9 NR4A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 AIFM3  
Affinity Capture-MS Homo sapiens
11 KIAA0226L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MARCH1  
Affinity Capture-MS Homo sapiens
14 GRK5 2869
Affinity Capture-MS Homo sapiens
15 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Biochemical Activity Homo sapiens
16 SNCG 6623
Biochemical Activity Homo sapiens
17 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CD6 923
Affinity Capture-MS Homo sapiens
19 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
21 PRSS23 11098
Two-hybrid Homo sapiens
22 RUFY1 80230
Affinity Capture-MS Homo sapiens
23 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
24 SHMT2 6472
Affinity Capture-RNA Homo sapiens
25 FSHR  
Biochemical Activity Homo sapiens
26 SMEK1 55671
Affinity Capture-MS Homo sapiens
27 KCNIP3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
28 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
29 CCDC85A  
Affinity Capture-MS Homo sapiens
30 C7orf31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 KCNE3  
Affinity Capture-MS Homo sapiens
32 RPA3 6119
Proximity Label-MS Homo sapiens
33 AFF4 27125
Affinity Capture-MS Homo sapiens
34 NOLC1 9221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DIEXF  
Affinity Capture-MS Homo sapiens
37 PNLIPRP2  
Affinity Capture-MS Homo sapiens
38 GRK4  
Affinity Capture-MS Homo sapiens
39 CCDC8  
Affinity Capture-MS Homo sapiens
40 DDB1 1642
Affinity Capture-Western Homo sapiens
41 ADNP 23394
Affinity Capture-MS Homo sapiens
42 TMCO1 54499
Cross-Linking-MS (XL-MS) Homo sapiens
43 SLC31A1 1317
Affinity Capture-MS Homo sapiens
44 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 GOPC 57120
Affinity Capture-MS Homo sapiens
47 EDAR  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GRK6 is involved
PathwayEvidenceSource
G alpha (s) signalling events TAS Reactome
GPCR downstream signalling TAS Reactome
Signal Transduction TAS Reactome
Signaling by GPCR TAS Reactome





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