Gene description for GRK6
Gene name G protein-coupled receptor kinase 6
Gene symbol GRK6
Other names/aliases GPRK6
Species Homo sapiens
 Database cross references - GRK6
ExoCarta ExoCarta_2870
Entrez Gene 2870
HGNC 4545
MIM 600869
UniProt P43250  
 GRK6 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GRK6
Molecular Function
    G-protein coupled receptor kinase activity GO:0004703 IEA
    beta-adrenergic receptor kinase activity GO:0047696 IMP
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
Biological Process
    Wnt signaling pathway GO:0016055 IEA
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 TAS
    termination of G-protein coupled receptor signaling pathway GO:0038032 IEA
    protein phosphorylation GO:0006468 IEA
Subcellular Localization
    membrane GO:0016020 IDA
 Experiment description of studies that identified GRK6 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GRK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRB2  
Invitro Homo sapiens
2 GIT1 28964
Invivo Homo sapiens
3 PRSS23 11098
Two-hybrid Homo sapiens
4 RCV1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 FSHR  
Biochemical Activity Homo sapiens
6 EDNRA  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GRK6 is involved
No pathways found





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