Gene description for KIF4A
Gene name kinesin family member 4A
Gene symbol KIF4A
Other names/aliases KIF4
KIF4G1
MRX100
Species Homo sapiens
 Database cross references - KIF4A
ExoCarta ExoCarta_24137
Entrez Gene 24137
HGNC 13339
MIM 300521
UniProt O95239  
 KIF4A identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
 Gene ontology annotations for KIF4A
Molecular Function
    DNA binding GO:0003677 IEA
    ATP binding GO:0005524 IEA
    microtubule motor activity GO:0003777 IEA
    microtubule binding GO:0008017 IEA
    ATPase activity GO:0016887 IBA
    protein binding GO:0005515 IPI
Biological Process
    anterograde axon cargo transport GO:0008089 TAS
    blood coagulation GO:0007596 TAS
    organelle organization GO:0006996 TAS
    microtubule-based movement GO:0007018 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    mitotic spindle midzone assembly GO:0051256 IMP
    metabolic process GO:0008152 TAS
    mitotic cytokinesis GO:0000281 IMP
    axon guidance GO:0007411 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 TAS
    spindle microtubule GO:0005876 TAS
    nucleoplasm GO:0005654 IDA
    chromosome GO:0005694 IEA
    midbody GO:0030496 IDA
    nuclear matrix GO:0016363 IEA
    kinesin complex GO:0005871 IBA
    membrane GO:0016020 IDA
 Experiment description of studies that identified KIF4A in exosomes
1
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HMG20B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 DNMT3B  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
3 DNPEP 23549
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KIF4A is involved
PathwayEvidenceSource
Kinesins TAS Reactome
MHC class II antigen presentation TAS Reactome
Recycling pathway of L1 TAS Reactome





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