Gene description for CDKN3
Gene name cyclin-dependent kinase inhibitor 3
Gene symbol CDKN3
Other names/aliases CDI1
CIP2
KAP
KAP1
Species Homo sapiens
 Database cross references - CDKN3
ExoCarta ExoCarta_1033
Vesiclepedia VP_1033
Entrez Gene 1033
HGNC 1791
MIM 123832
UniProt Q16667  
 CDKN3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CDKN3
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IDA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine/serine/threonine phosphatase activity GO:0008138 TAS
    myosin phosphatase activity GO:0017018 IEA
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    negative regulation of cell population proliferation GO:0008285 TAS
    regulation of cell cycle GO:0051726 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified CDKN3 in exosomes
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CDKN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
2 NAP1L4 4676
Affinity Capture-MS Homo sapiens
3 SEC63 11231
Affinity Capture-MS Homo sapiens
4 CST8  
Affinity Capture-MS Homo sapiens
5 CDC42BPB 9578
Affinity Capture-Western Homo sapiens
6 ROCK1 6093
Affinity Capture-Western Homo sapiens
7 RAD23A 5886
Affinity Capture-MS Homo sapiens
8 TP53BP1 7158
Affinity Capture-MS Homo sapiens
9 NOL11  
Affinity Capture-MS Homo sapiens
10 R3HCC1L  
Affinity Capture-MS Homo sapiens
11 XRCC4  
Affinity Capture-MS Homo sapiens
12 C18orf25 147339
Affinity Capture-MS Homo sapiens
13 DIS3L  
Affinity Capture-MS Homo sapiens
14 HRNR 388697
Affinity Capture-MS Homo sapiens
15 RAD23B 5887
Affinity Capture-MS Homo sapiens
16 BRCA1 672
Affinity Capture-MS Homo sapiens
17 CD47 961
Affinity Capture-MS Homo sapiens
18 ROCK2 9475
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
19 PTPN5  
Affinity Capture-MS Homo sapiens
20 CDK1 983
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 MS4A3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
22 BAG6 7917
Affinity Capture-MS Homo sapiens
23 CECR5 27440
Affinity Capture-MS Homo sapiens
24 CDK2 1017
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
25 PPP1R12A 4659
Biochemical Activity Homo sapiens
26 C22orf29  
Affinity Capture-MS Homo sapiens
27 WWC1  
Affinity Capture-MS Homo sapiens
28 CDKN3 1033
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 JMY  
Affinity Capture-MS Homo sapiens
30 RMND5A 64795
Affinity Capture-Western Homo sapiens
31 STMN1 3925
Affinity Capture-MS Homo sapiens
32 BMI1  
Affinity Capture-Western Homo sapiens
33 HAX1  
Affinity Capture-MS Homo sapiens
34 NCOA3  
Two-hybrid Homo sapiens
35 DLAT 1737
Affinity Capture-MS Homo sapiens
36 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
37 ARHGEF28  
Affinity Capture-MS Homo sapiens
38 CLPB 81570
Affinity Capture-MS Homo sapiens
39 GOPC 57120
Affinity Capture-MS Homo sapiens
40 HLCS  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CDKN3 is involved
No pathways found





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