Gene description for NDST1
Gene name N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
Gene symbol NDST1
Other names/aliases HSST
MRT46
NST1
Species Homo sapiens
 Database cross references - NDST1
ExoCarta ExoCarta_3340
Vesiclepedia VP_3340
Entrez Gene 3340
HGNC 7680
MIM 600853
UniProt P52848  
 NDST1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NDST1
Molecular Function
    protein binding GO:0005515 IPI
    [heparan sulfate]-glucosamine N-sulfotransferase activity GO:0015016 IBA
    [heparan sulfate]-glucosamine N-sulfotransferase activity GO:0015016 IDA
    [heparan sulfate]-glucosamine N-sulfotransferase activity GO:0015016 IMP
    [heparan sulfate]-glucosamine N-sulfotransferase activity GO:0015016 TAS
    deacetylase activity GO:0019213 IBA
    N-acetylglucosamine deacetylase activity GO:0050119 TAS
    heparan sulfate N-deacetylase activity GO:0102140 IDA
    heparan sulfate N-deacetylase activity GO:0102140 ISS
Biological Process
    cardiac septum development GO:0003279 IEA
    inflammatory response GO:0006954 IEA
    respiratory gaseous exchange by respiratory system GO:0007585 IEA
    cell population proliferation GO:0008283 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 IBA
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 ISS
    heparan sulfate proteoglycan biosynthetic process, enzymatic modification GO:0015015 TAS
    heparin biosynthetic process GO:0030210 IEA
    forebrain development GO:0030900 IEA
    midbrain development GO:0030901 IEA
    aorta development GO:0035904 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    embryonic neurocranium morphogenesis GO:0048702 IEA
    embryonic viscerocranium morphogenesis GO:0048703 IEA
    coronary vasculature development GO:0060976 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IBA
    trans-Golgi network membrane GO:0032588 IDA
 Experiment description of studies that identified NDST1 in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 CPEB4  
Co-fractionation Homo sapiens
3 NRROS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
6 NDST2  
Affinity Capture-MS Homo sapiens
7 LCN6  
Affinity Capture-MS Homo sapiens
8 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NDST4  
Affinity Capture-MS Homo sapiens
10 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HPN  
Affinity Capture-MS Homo sapiens
12 GALNT10 55568
Affinity Capture-MS Homo sapiens
13 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PDGFRA 5156
Affinity Capture-MS Homo sapiens
15 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RLN1  
Affinity Capture-MS Homo sapiens
17 CLEC12B  
Affinity Capture-MS Homo sapiens
18 TMEM132A 54972
Affinity Capture-MS Homo sapiens
19 TMEM106A  
Affinity Capture-MS Homo sapiens
20 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 Sidt2  
Affinity Capture-MS Mus musculus
22 SCGB2A2  
Affinity Capture-MS Homo sapiens
23 C1orf54  
Affinity Capture-MS Homo sapiens
24 CEACAM8 1088
Affinity Capture-MS Homo sapiens
25 Brca1  
Affinity Capture-MS Mus musculus
26 BAGE2  
Affinity Capture-MS Homo sapiens
27 ST14 6768
Affinity Capture-MS Homo sapiens
28 XRCC5 7520
Affinity Capture-MS Homo sapiens
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