Gene description for NDST1
Gene name N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
Gene symbol NDST1
Other names/aliases HSST
MRT46
NST1
Species Homo sapiens
 Database cross references - NDST1
ExoCarta ExoCarta_3340
Entrez Gene 3340
HGNC 7680
MIM 600853
UniProt P52848  
 NDST1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NDST1
Molecular Function
    deacetylase activity GO:0019213 IEA
    [heparan sulfate]-glucosamine N-sulfotransferase activity GO:0015016 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    embryonic viscerocranium morphogenesis GO:0048703 IEA
    MAPK cascade GO:0000165 IEA
    polysaccharide biosynthetic process GO:0000271 IEA
    respiratory gaseous exchange GO:0007585 IEA
    smoothened signaling pathway GO:0007224 IEA
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IEA
    embryonic neurocranium morphogenesis GO:0048702 IEA
    small molecule metabolic process GO:0044281 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    midbrain development GO:0030901 IEA
    heparin biosynthetic process GO:0030210 IEA
    forebrain development GO:0030900 IEA
    inflammatory response GO:0006954 IEA
    glycosaminoglycan biosynthetic process GO:0006024 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    Golgi membrane GO:0000139 TAS
 Experiment description of studies that identified NDST1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NDST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NDST1 is involved
PathwayEvidenceSource
HS-GAG biosynthesis TAS Reactome





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