Gene description for DHX58
Gene name DEXH (Asp-Glu-X-His) box polypeptide 58
Gene symbol DHX58
Other names/aliases D11LGP2
D11lgp2e
LGP2
RLR-3
Species Homo sapiens
 Database cross references - DHX58
ExoCarta ExoCarta_79132
Vesiclepedia VP_79132
Entrez Gene 79132
HGNC 29517
MIM 608588
UniProt Q96C10  
 DHX58 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for DHX58
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IEA
    RNA helicase activity GO:0003724 IMP
    double-stranded RNA binding GO:0003725 IBA
    double-stranded RNA binding GO:0003725 IDA
    single-stranded RNA binding GO:0003727 IBA
    single-stranded RNA binding GO:0003727 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
    ATP hydrolysis activity GO:0016887 IMP
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 IBA
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 IMP
    chromatin remodeling GO:0006338 IEA
    response to virus GO:0009615 ISS
    response to bacterium GO:0009617 IEA
    negative regulation of type I interferon production GO:0032480 IDA
    positive regulation of type I interferon production GO:0032481 ISS
    negative regulation of MDA-5 signaling pathway GO:0039534 IMP
    negative regulation of RIG-I signaling pathway GO:0039536 IBA
    negative regulation of RIG-I signaling pathway GO:0039536 IDA
    negative regulation of RIG-I signaling pathway GO:0039536 IMP
    regulation of innate immune response GO:0045088 IDA
    negative regulation of innate immune response GO:0045824 IDA
    antiviral innate immune response GO:0140374 IBA
    chromatin looping GO:0140588 IEA
    positive regulation of MDA-5 signaling pathway GO:1900245 ISS
    positive regulation of RIG-I signaling pathway GO:1900246 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified DHX58 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHX58
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 DICER1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 EIF2AK2 5610
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 CDKN2AIP  
Affinity Capture-MS Homo sapiens
6 XRN2 22803
Affinity Capture-MS Homo sapiens
7 NKRF 55922
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 AP2B1 163
Two-hybrid Homo sapiens
9 STAU1 6780
Affinity Capture-MS Homo sapiens
10 USP21  
Affinity Capture-Western Homo sapiens
11 STAU2 27067
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 DHX30 22907
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
13 EIF6 3692
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
14 AGO2 27161
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which DHX58 is involved
PathwayEvidenceSource
DDX58/IFIH1-mediated induction of interferon-alpha/beta TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome





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