Gene description for AKT1S1
Gene name AKT1 substrate 1 (proline-rich)
Gene symbol AKT1S1
Other names/aliases Lobe
PRAS40
Species Homo sapiens
 Database cross references - AKT1S1
ExoCarta ExoCarta_84335
Entrez Gene 84335
HGNC 28426
MIM 610221
UniProt Q96B36  
 AKT1S1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for AKT1S1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    cellular response to heat GO:0034605 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    regulation of neuron apoptotic process GO:0043523 ISS
    innate immune response GO:0045087 TAS
    negative regulation of cell size GO:0045792 IDA
    regulation of cellular response to heat GO:1900034 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    negative regulation of protein kinase activity GO:0006469 IDA
    negative regulation of TOR signaling GO:0032007 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
Subcellular Localization
    TORC1 complex GO:0031931 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified AKT1S1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AKT1S1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF6 3692
Two-hybrid Homo sapiens
2 TCEA2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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