Gene description for NKAP
Gene name NFKB activating protein
Gene symbol NKAP
Other names/aliases -
Species Homo sapiens
 Database cross references - NKAP
ExoCarta ExoCarta_79576
Vesiclepedia VP_79576
Entrez Gene 79576
HGNC 29873
MIM 300766
UniProt Q8N5F7  
 NKAP identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NKAP
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    chromatin DNA binding GO:0031490 IEA
    chromatin binding GO:0003682 IDA
Biological Process
    granulocyte differentiation GO:0030851 IEA
    stem cell maintenance GO:0019827 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    Notch signaling pathway GO:0007219 IEA
    T cell differentiation in thymus GO:0033077 IEA
    hematopoietic stem cell proliferation GO:0071425 IEA
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of alpha-beta T cell differentiation GO:0046638 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified NKAP in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NKAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LUC7L2 51631
Two-hybrid Homo sapiens
2 THAP1  
Two-hybrid Homo sapiens
3 Cep70  
Two-hybrid Homo sapiens
4 FLJ30707  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NKAP is involved
No pathways found





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