Gene description for NKAP
Gene name NFKB activating protein
Gene symbol NKAP
Other names/aliases -
Species Homo sapiens
 Database cross references - NKAP
ExoCarta ExoCarta_79576
Vesiclepedia VP_79576
Entrez Gene 79576
HGNC 29873
MIM 300766
UniProt Q8N5F7  
 NKAP identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NKAP
Molecular Function
    chromatin binding GO:0003682 IBA
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    chromatin DNA binding GO:0031490 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    Notch signaling pathway GO:0007219 IEA
    granulocyte differentiation GO:0030851 IEA
    T cell differentiation in thymus GO:0033077 IEA
    somatic stem cell population maintenance GO:0035019 IEA
    negative regulation of DNA-templated transcription GO:0045892 IBA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of alpha-beta T cell differentiation GO:0046638 IMP
    hematopoietic stem cell proliferation GO:0071425 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified NKAP in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NKAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SNRPA 6626
Affinity Capture-MS Homo sapiens
3 RSBN1L  
Affinity Capture-MS Homo sapiens
4 UBE2O 63893
Affinity Capture-MS Homo sapiens
5 NOS1AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ZC3H14  
Affinity Capture-MS Homo sapiens
7 NOP56 10528
Proximity Label-MS Homo sapiens
8 DHX8 1659
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 CHCHD1  
Proximity Label-MS Homo sapiens
10 BRD2  
Affinity Capture-MS Homo sapiens
11 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PRPF3  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
13 CBY1  
Affinity Capture-MS Homo sapiens
14 PRPF40A 55660
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
15 ANKRD50 57182
Affinity Capture-MS Homo sapiens
16 CHCHD4  
Affinity Capture-MS Homo sapiens
17 DDX23 9416
Proximity Label-MS Homo sapiens
18 AKAP17A  
Affinity Capture-MS Homo sapiens
19 CEP70  
Two-hybrid Homo sapiens
20 CDK11B 984
Co-fractionation Homo sapiens
21 FRA10AC1 118924
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 ING3  
Affinity Capture-MS Homo sapiens
23 SRRM2 23524
Affinity Capture-MS Homo sapiens
24 SNRPC 6631
Affinity Capture-MS Homo sapiens
25 NKAPL 222698
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 U2AF1 7307
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
27 DDX41 51428
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CST1  
Affinity Capture-MS Homo sapiens
29 YWHAG 7532
Affinity Capture-MS Homo sapiens
30 MYCN  
Affinity Capture-MS Homo sapiens
31 USP7 7874
Affinity Capture-MS Homo sapiens
32 CSNK2B 1460
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 DYNLT1 6993
Proximity Label-MS Homo sapiens
34 NINL  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CST2  
Affinity Capture-MS Homo sapiens
36 EPB41L3 23136
Affinity Capture-MS Homo sapiens
37 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 POLD1 5424
Affinity Capture-MS Homo sapiens
39 ARMC8 25852
Affinity Capture-MS Homo sapiens
40 ACIN1 22985
Affinity Capture-MS Homo sapiens
41 C12orf49  
Negative Genetic Homo sapiens
42 ZCCHC17  
Affinity Capture-MS Homo sapiens
43 SNIP1  
Affinity Capture-MS Homo sapiens
44 FAM124A  
Two-hybrid Homo sapiens
45 OGT 8473
Reconstituted Complex Homo sapiens
46 RAD18  
Affinity Capture-MS Homo sapiens
47 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
48 RBMX2  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
49 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
50 LCN1 3933
Affinity Capture-MS Homo sapiens
51 LYAR 55646
Affinity Capture-MS Homo sapiens
52 HECTD1 25831
Affinity Capture-MS Homo sapiens
53 NKTR  
Affinity Capture-MS Homo sapiens
54 SRPK1 6732
Biochemical Activity Homo sapiens
55 CHD9 80205
Affinity Capture-MS Homo sapiens
56 PCBP2 5094
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
57 RBM39 9584
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 C11orf57  
Affinity Capture-MS Homo sapiens
59 RC3H2  
Affinity Capture-MS Homo sapiens
60 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SNRPB 6628
Affinity Capture-MS Homo sapiens
62 C1orf226  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 NUP43 348995
Proximity Label-MS Homo sapiens
64 THAP1  
Two-hybrid Homo sapiens
65 AP2M1 1173
Affinity Capture-MS Homo sapiens
66 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
67 CST4 1472
Affinity Capture-MS Homo sapiens
68 AQR 9716
Affinity Capture-MS Homo sapiens
69 MYC  
Affinity Capture-MS Homo sapiens
70 ZC3H18  
Affinity Capture-MS Homo sapiens
71 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 MYEF2 50804
Affinity Capture-MS Homo sapiens
73 UPF3B 65109
Affinity Capture-MS Homo sapiens
74 DDRGK1 65992
Affinity Capture-MS Homo sapiens
75 Ddx41  
Affinity Capture-MS Mus musculus
76 BPIFA2 140683
Affinity Capture-MS Homo sapiens
77 HIST1H1T 3010
Affinity Capture-MS Homo sapiens
78 BRD3 8019
Affinity Capture-MS Homo sapiens
79 NKAP 79576
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 CRNN 49860
Affinity Capture-MS Homo sapiens
81 FTL 2512
Affinity Capture-MS Homo sapiens
82 DHX15 1665
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
83 LLGL2 3993
Affinity Capture-MS Homo sapiens
84 CPSF6 11052
Affinity Capture-MS Homo sapiens
85 RBM25 58517
Affinity Capture-MS Homo sapiens
86 MDK 4192
Affinity Capture-MS Homo sapiens
87 HECTD3 79654
Affinity Capture-MS Homo sapiens
88 PPIG 9360
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
89 CUL5 8065
Affinity Capture-MS Homo sapiens
90 TTC14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
91 SQSTM1 8878
Proximity Label-MS Homo sapiens
92 EPB41L2 2037
Affinity Capture-MS Homo sapiens
93 BUB3 9184
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
94 CHMP4C 92421
Affinity Capture-MS Homo sapiens
95 SREK1IP1  
Affinity Capture-MS Homo sapiens
96 ZCCHC10  
Affinity Capture-MS Homo sapiens
97 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 TOPORS  
Affinity Capture-MS Homo sapiens
99 C9orf72  
Affinity Capture-MS Homo sapiens
100 RC3H1 149041
Affinity Capture-MS Homo sapiens
101 LUC7L 55692
Affinity Capture-MS Homo sapiens
102 ZZEF1 23140
Affinity Capture-MS Homo sapiens
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