Gene description for DDX41
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
Gene symbol DDX41
Other names/aliases ABS
Species Homo sapiens
 Database cross references - DDX41
ExoCarta ExoCarta_51428
Vesiclepedia VP_51428
Entrez Gene 51428
HGNC 18674
MIM 608170
UniProt Q9UJV9  
 DDX41 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DDX41
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    metal ion binding GO:0046872 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IMP
    chromatin remodeling GO:0006338 IEA
    apoptotic process GO:0006915 TAS
    cell population proliferation GO:0008283 IMP
    cell differentiation GO:0030154 IMP
    chromatin looping GO:0140588 IEA
    cGAS/STING signaling pathway GO:0140896 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IBA
    spliceosomal complex GO:0005681 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified DDX41 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DDX41
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 SLFN11 91607
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 KLC2 64837
Two-hybrid Homo sapiens
6 COIL  
Proximity Label-MS Homo sapiens
7 NOS1AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DHX8 1659
Proximity Label-MS Homo sapiens
9 CHCHD1  
Proximity Label-MS Homo sapiens
10 BRD2  
Affinity Capture-MS Homo sapiens
11 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CREM  
Affinity Capture-MS Homo sapiens
13 CBY1  
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 ANKRD50 57182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 USP36  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DDX23 9416
Proximity Label-MS Homo sapiens
18 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CEP70  
Two-hybrid Homo sapiens
20 CHAF1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 KIF23 9493
Affinity Capture-MS Homo sapiens
22 Tube1  
Affinity Capture-MS Mus musculus
23 FBXW7  
Affinity Capture-MS Homo sapiens
24 ARID4B  
Affinity Capture-MS Homo sapiens
25 SRRM2 23524
Affinity Capture-MS Homo sapiens
26 NKAPL 222698
Two-hybrid Homo sapiens
27 OBSL1 23363
Affinity Capture-MS Homo sapiens
28 MECP2 4204
Affinity Capture-MS Homo sapiens
29 MYCN  
Affinity Capture-MS Homo sapiens
30 FAM20C 56975
Affinity Capture-MS Homo sapiens
31 DVL2 1856
Affinity Capture-MS Homo sapiens
32 DYNLT1 6993
Proximity Label-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 LIN28A  
Affinity Capture-MS Homo sapiens
35 UFL1 23376
Affinity Capture-MS Homo sapiens
36 SRP9 6726
Affinity Capture-MS Homo sapiens
37 JMJD6 23210
Affinity Capture-MS Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 GZMH  
Affinity Capture-MS Homo sapiens
41 IKZF3  
Affinity Capture-MS Homo sapiens
42 CHMP4B 128866
Affinity Capture-MS Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 RPL31 6160
Proximity Label-MS Homo sapiens
45 IFI16 3428
Affinity Capture-MS Homo sapiens
46 NCL 4691
Affinity Capture-MS Homo sapiens
47 CEBPA  
Protein-peptide Homo sapiens
48 OGT 8473
Reconstituted Complex Homo sapiens
49 PITRM1 10531
Co-fractionation Homo sapiens
50 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SNRPB 6628
Affinity Capture-MS Homo sapiens
52 CUL3 8452
Affinity Capture-MS Homo sapiens
53 RPS6 6194
Proximity Label-MS Homo sapiens
54 Csde1 229663
Affinity Capture-MS Mus musculus
55 IKZF1  
Affinity Capture-MS Homo sapiens
56 BRMS1L  
Affinity Capture-MS Homo sapiens
57 NXF1 10482
Affinity Capture-RNA Homo sapiens
58 CEP250 11190
Affinity Capture-MS Homo sapiens
59 MYC  
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SNW1 22938
Affinity Capture-MS Homo sapiens
63 DDRGK1 65992
Affinity Capture-MS Homo sapiens
64 Ddx41  
Affinity Capture-MS Mus musculus
65 BRD3 8019
Affinity Capture-MS Homo sapiens
66 NKAP 79576
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
68 PARP1 142
Proximity Label-MS Homo sapiens
69 WDR77 79084
Affinity Capture-MS Homo sapiens
70 SIN3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TUBG1 7283
Two-hybrid Homo sapiens
72 TBC1D9  
Affinity Capture-MS Homo sapiens
73 MEN1 4221
Affinity Capture-MS Homo sapiens
74 SNRPA 6626
Affinity Capture-MS Homo sapiens
75 ARL8A 127829
Affinity Capture-MS Homo sapiens
76 SHMT2 6472
Affinity Capture-RNA Homo sapiens
77 DDX58 23586
Affinity Capture-RNA Homo sapiens
78 CLVS2 134829
Co-fractionation Homo sapiens
79 DHX40  
Proximity Label-MS Homo sapiens
80 MOV10 4343
Affinity Capture-RNA Homo sapiens
81 PSMA5 5686
Affinity Capture-MS Homo sapiens
82 PINK1  
Affinity Capture-MS Homo sapiens
83 TCAF1 9747
Affinity Capture-MS Homo sapiens
84 CIT 11113
Affinity Capture-MS Homo sapiens
85 AIDA 64853
Two-hybrid Homo sapiens
86 KRAS 3845
Negative Genetic Homo sapiens
87 C9orf72  
Affinity Capture-MS Homo sapiens
88 RC3H1 149041
Affinity Capture-MS Homo sapiens
89 Vps41  
Affinity Capture-MS Mus musculus
90 EP300 2033
Affinity Capture-MS Homo sapiens
91 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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