Gene description for SMG9
Gene name SMG9 nonsense mediated mRNA decay factor
Gene symbol SMG9
Other names/aliases C19orf61
F17127_1
Species Homo sapiens
 Database cross references - SMG9
ExoCarta ExoCarta_56006
Vesiclepedia VP_56006
Entrez Gene 56006
HGNC 25763
MIM 613176
UniProt Q9H0W8  
 SMG9 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMG9
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IBA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IMP
    eye development GO:0001654 ISS
    in utero embryonic development GO:0001701 IEA
    brain development GO:0007420 ISS
    heart development GO:0007507 ISS
    negative regulation of apoptotic process GO:0043066 EXP
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SMG9 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMG9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTAG1A  
Two-hybrid Homo sapiens
2 PHF1  
Two-hybrid Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 SERBP1 26135
Affinity Capture-MS Homo sapiens
5 SIRT6  
Affinity Capture-MS Homo sapiens
6 SARS 6301
Co-fractionation Homo sapiens
7 PFDN5 5204
Two-hybrid Homo sapiens
8 AK8  
Two-hybrid Homo sapiens
9 RBM8A 9939
Affinity Capture-Western Homo sapiens
10 SLC17A9  
Two-hybrid Homo sapiens
11 HSF2BP  
Two-hybrid Homo sapiens
12 SMG8 55181
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
13 NFKBID  
Two-hybrid Homo sapiens
14 CCDC57 284001
Two-hybrid Homo sapiens
15 CTAG1B  
Two-hybrid Homo sapiens
16 VPRBP 9730
Affinity Capture-MS Homo sapiens
17 PRKAR1B  
Two-hybrid Homo sapiens
18 KRT34  
Two-hybrid Homo sapiens
19 SQSTM1 8878
Proximity Label-MS Homo sapiens
20 GSG1  
Two-hybrid Homo sapiens
21 PARK2  
Affinity Capture-MS Homo sapiens
22 TSC1 7248
Two-hybrid Homo sapiens
23 PRPSAP1 5635
Affinity Capture-MS Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 ATG4C  
Affinity Capture-MS Homo sapiens
26 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
27 ZNF655  
Two-hybrid Homo sapiens
28 INCA1  
Two-hybrid Homo sapiens
29 GOLGA2 2801
Two-hybrid Homo sapiens
30 SYP  
Two-hybrid Homo sapiens
31 AGTRAP 57085
Two-hybrid Homo sapiens
32 DDX11 1663
Affinity Capture-MS Homo sapiens
33 PLEKHG4  
Two-hybrid Homo sapiens
34 TCF4  
Two-hybrid Homo sapiens
35 PNMA3  
Two-hybrid Homo sapiens
36 RPA3 6119
Proximity Label-MS Homo sapiens
37 TRIM23 373
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 RASSF10  
Two-hybrid Homo sapiens
39 UPF1 5976
Affinity Capture-Western Homo sapiens
40 CMTM4  
Two-hybrid Homo sapiens
41 TRIM27  
Two-hybrid Homo sapiens
42 SMG1 23049
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
43 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 LDLR 3949
Positive Genetic Homo sapiens
45 FNTB 2342
Two-hybrid Homo sapiens
46 SLC50A1 55974
Two-hybrid Homo sapiens
47 CEP76  
Two-hybrid Homo sapiens
48 DGAT2L6  
Two-hybrid Homo sapiens
49 MTUS2 23281
Two-hybrid Homo sapiens
50 KRTAP10-8  
Two-hybrid Homo sapiens
51 KRT31 3881
Two-hybrid Homo sapiens
52 REL 5966
Two-hybrid Homo sapiens
53 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ARFIP2 23647
Two-hybrid Homo sapiens
View the network image/svg+xml



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