Gene description for PLEKHF1
Gene name pleckstrin homology domain containing, family F (with FYVE domain) member 1
Gene symbol PLEKHF1
Other names/aliases APPD
LAPF
PHAFIN1
ZFYVE15
Species Homo sapiens
 Database cross references - PLEKHF1
ExoCarta ExoCarta_79156
Entrez Gene 79156
HGNC 20764
MIM 615200
UniProt Q96S99  
 PLEKHF1 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for PLEKHF1
Molecular Function
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    phosphatidylinositol-5-phosphate binding GO:0010314 IDA
Biological Process
    vesicle organization GO:0016050 IMP
    apoptotic process GO:0006915 IEA
    positive regulation of autophagy GO:0010508 IDA
    protein localization to plasma membrane GO:0072659 IMP
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
    endosome organization GO:0007032 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 IC
    lysosome GO:0005764 IDA
    endosome GO:0005768 IDA
    endosome membrane GO:0010008 IC
    nucleus GO:0005634 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PLEKHF1 in exosomes
1
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLEKHF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF1BP 26128
Two-hybrid Homo sapiens
2 TNNT1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PLEKHF1 is involved
No pathways found





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