Gene description for PTBP3
Gene name polypyrimidine tract binding protein 3
Gene symbol PTBP3
Other names/aliases ROD1
Species Homo sapiens
 Database cross references - PTBP3
ExoCarta ExoCarta_9991
Vesiclepedia VP_9991
Entrez Gene 9991
HGNC 10253
MIM 607527
UniProt O95758  
 PTBP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PTBP3
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    anatomical structure morphogenesis GO:0009653 TAS
    negative regulation of RNA splicing GO:0033119 IDA
    erythrocyte maturation GO:0043249 IEA
    regulation of RNA splicing GO:0043484 IBA
    regulation of cell differentiation GO:0045595 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
 Experiment description of studies that identified PTBP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTBP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 RALY 22913
Affinity Capture-MS Homo sapiens
3 RBMXL1  
Affinity Capture-MS Homo sapiens
4 HNRNPC 3183
Affinity Capture-MS Homo sapiens
5 RSL1D1 26156
Affinity Capture-MS Homo sapiens
6 RBPMS 11030
Two-hybrid Homo sapiens
7 COIL  
Proximity Label-MS Homo sapiens
8 PPIE 10450
Affinity Capture-MS Homo sapiens
9 LARP1B 55132
Affinity Capture-MS Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 PUM2 23369
Affinity Capture-MS Homo sapiens
12 NENF 29937
Co-fractionation Homo sapiens
13 RPS2P32  
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 MPHOSPH10 10199
Affinity Capture-MS Homo sapiens
17 ILF2 3608
Co-fractionation Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 PRKCDBP 112464
Co-fractionation Homo sapiens
20 DDX24 57062
Affinity Capture-MS Homo sapiens
21 D2HGDH 728294
Affinity Capture-MS Homo sapiens
22 COPB2 9276
Affinity Capture-MS Homo sapiens
23 CDH2 1000
Co-fractionation Homo sapiens
24 PRC1 9055
Affinity Capture-MS Homo sapiens
25 NCOA5  
Affinity Capture-MS Homo sapiens
26 Ankrd26  
Affinity Capture-MS Mus musculus
27 STAU2 27067
Affinity Capture-MS Homo sapiens
28 PRKRA 8575
Affinity Capture-MS Homo sapiens
29 ATG16L1 55054
Affinity Capture-MS Homo sapiens
30 SLC25A31 83447
Affinity Capture-MS Homo sapiens
31 MECP2 4204
Affinity Capture-MS Homo sapiens
32 FAM120A 23196
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 AGO2 27161
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 APOO 79135
Proximity Label-MS Homo sapiens
36 E2F4  
Affinity Capture-MS Homo sapiens
37 FAM96A  
Affinity Capture-MS Homo sapiens
38 SULT1C4  
Affinity Capture-MS Homo sapiens
39 RBM28 55131
Affinity Capture-MS Homo sapiens
40 PHAX  
Affinity Capture-MS Homo sapiens
41 COPE 11316
Affinity Capture-MS Homo sapiens
42 C17orf85  
Affinity Capture-MS Homo sapiens
43 Taf15  
Two-hybrid Mus musculus
44 STAU1 6780
Affinity Capture-MS Homo sapiens
45 MKRN2 23609
Affinity Capture-MS Homo sapiens
46 CHEK1  
Co-fractionation Homo sapiens
47 TRIM11  
Affinity Capture-MS Homo sapiens
48 Cpeb4  
Two-hybrid Mus musculus
49 Ptbp1 19205
Affinity Capture-MS Mus musculus
50 PICALM 8301
Co-fractionation Homo sapiens
51 DDX27 55661
Affinity Capture-MS Homo sapiens
52 UBXN6 80700
Affinity Capture-MS Homo sapiens
53 NMNAT2  
Affinity Capture-MS Homo sapiens
54 TXN2 25828
Co-fractionation Homo sapiens
55 PSG11  
Affinity Capture-MS Homo sapiens
56 ELAVL2 1993
Affinity Capture-MS Homo sapiens
57 SF3B2 10992
Affinity Capture-MS Homo sapiens
58 CLK3  
Affinity Capture-MS Homo sapiens
59 KIF20A 10112
Affinity Capture-MS Homo sapiens
60 BAG2 9532
Affinity Capture-MS Homo sapiens
61 YBX1 4904
Affinity Capture-MS Homo sapiens
62 CD2BP2 10421
Two-hybrid Homo sapiens
63 METTL21B  
Affinity Capture-MS Homo sapiens
64 ZC3H11A  
Affinity Capture-MS Homo sapiens
65 Pcbp1 23983
Two-hybrid Mus musculus
66 NIP7 51388
Affinity Capture-MS Homo sapiens
67 YTHDC1  
Affinity Capture-MS Homo sapiens
68 PRR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 C1RL 51279
Affinity Capture-MS Homo sapiens
70 PCBP1 5093
Affinity Capture-MS Homo sapiens
71 IFI35 3430
Affinity Capture-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 PPWD1 23398
Co-fractionation Homo sapiens
74 PABPC5 140886
Affinity Capture-MS Homo sapiens
75 CLASRP  
Affinity Capture-MS Homo sapiens
76 MATR3 9782
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 TAS2R39  
Affinity Capture-MS Homo sapiens
78 BMI1  
Affinity Capture-MS Homo sapiens
79 FYTTD1  
Affinity Capture-MS Homo sapiens
80 UGT1A8  
Affinity Capture-MS Homo sapiens
81 FTSJ3 117246
Affinity Capture-MS Homo sapiens
82 LGALSL 29094
Co-fractionation Homo sapiens
83 CLPP 8192
Proximity Label-MS Homo sapiens
84 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
85 RBM45  
Affinity Capture-MS Homo sapiens
86 ILF3 3609
Co-fractionation Homo sapiens
87 SRRM1 10250
Affinity Capture-MS Homo sapiens
88 CALU 813
Co-fractionation Homo sapiens
89 PPHLN1  
Affinity Capture-MS Homo sapiens
90 CCT8L2  
Affinity Capture-MS Homo sapiens
91 PTBP1 5725
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
92 SQSTM1 8878
Proximity Label-MS Homo sapiens
93 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
94 PTBP2  
Cross-Linking-MS (XL-MS) Homo sapiens
95 SUGP2  
Affinity Capture-MS Homo sapiens
96 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 UPF1 5976
Affinity Capture-MS Homo sapiens
98 SSSCA1 10534
Affinity Capture-MS Homo sapiens
99 CIT 11113
Affinity Capture-MS Homo sapiens
100 SERBP1 26135
Affinity Capture-MS Homo sapiens
101 CIZ1  
Affinity Capture-MS Homo sapiens
102 RC3H1 149041
Co-fractionation Homo sapiens
103 CCR1  
Affinity Capture-MS Homo sapiens
104 NOTCH1 4851
Affinity Capture-MS Homo sapiens
105 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PTBP3 is involved
No pathways found





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