Gene description for NKD2
Gene name naked cuticle homolog 2 (Drosophila)
Gene symbol NKD2
Other names/aliases Naked2
Species Homo sapiens
 Database cross references - NKD2
ExoCarta ExoCarta_85409
Vesiclepedia VP_85409
Entrez Gene 85409
HGNC 17046
MIM 607852
UniProt Q969F2  
 NKD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NKD2
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    growth factor binding GO:0019838 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    myosin heavy chain binding GO:0032036 IPI
    ATPase binding GO:0051117 IPI
    molecular adaptor activity GO:0060090 EXP
Biological Process
    exocytosis GO:0006887 IEA
    positive regulation of protein processing GO:0010954 IDA
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of Wnt signaling pathway GO:0030178 IBA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    Golgi vesicle fusion to target membrane GO:0048210 IMP
    protein localization to plasma membrane GO:0072659 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IDA
    basolateral plasma membrane GO:0016323 IDA
    lateral plasma membrane GO:0016328 IDA
    cytoplasmic vesicle GO:0031410 IDA
    exocytic vesicle GO:0070382 IDA
    cell periphery GO:0071944 IDA
 Experiment description of studies that identified NKD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NKD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF43  
Proximity Label-MS Homo sapiens
2 DVL1 1855
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
3 PLS1 5357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FUS 2521
Two-hybrid Homo sapiens
5 TNFRSF1B  
Affinity Capture-MS Homo sapiens
6 GRB2 2885
Two-hybrid Homo sapiens
7 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 UBE2E3 10477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RNF25 64320
Affinity Capture-Western Homo sapiens
11 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RCHY1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
13 ANAPC11  
Two-hybrid Homo sapiens
14 CDKN1A  
Two-hybrid Homo sapiens
15 VDR  
Two-hybrid Homo sapiens
16 UBE2D2 7322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CDH1 999
Co-fractionation Homo sapiens
18 PMVK 10654
Affinity Capture-MS Homo sapiens
19 SLX4  
Affinity Capture-MS Homo sapiens
20 TGFA  
Affinity Capture-Western Homo sapiens
21 YWHAQ 10971
Affinity Capture-MS Homo sapiens
22 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ARMT1 79624
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MARCH5  
Affinity Capture-MS Homo sapiens
25 CAV1 857
Co-fractionation Homo sapiens
26 LNX1  
Reconstituted Complex Homo sapiens
27 CHMP4A 29082
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NKD2 is involved
No pathways found





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